Maxim Baranov Ins and outs of antigen processing by dendritic cells: from antigen sampling, uptake, degradation to presentation
This dissertation summarizes complex mechanisms of antigen uptake and processing by dendritic cells (DCs).read more
Maxim Baranov Ins and outs of antigen processing by dendritic cells: from antigen sampling, uptake, degradation to presentationDendritic cells (DC) of the immune system can take up unwanted pathogens or cancer cells though phagocytosis. Phagocytosis is done by membrane structures that can capture pathogenic particles in a ‘digestive’ lipid envelope. The lipid composition of the membrane wrap determines the efficiency of phagocytosis. Phosphatidyl inositol phosphates (PI) allow them to bind specific proteins that float freely in the cell. In this thesis, PI(3,4)P2 binding protein SWAP70 has been identified. This protein can be found in large quantities on early phagosomes and is necessary for successful antigen uptake. Furthermore, the role of the phosphoinositide kinase PIKfyve that can produce the PI type PI(3,5)P2 was identified, which is crucial for membrane fusion of phagosomes with lysosomes for ‘digestion’. The experiments also revealed that DCs with podosomes, analogous to arms, can capture pathogens without having to migrate all the way through the layer endothelial/epithelial cells. This dissertation summarizes complex mechanisms of antigen uptake and processing by DCs.
Date, time and location PhD defense
- Date: 4 September 2019
- Time: 10:30 hrs
- Location: Radboud Universiteit, Academiezaal Aula, Comeniuslaan 2
In 2014, Maxim Baranov (1987) started a PhD research described in his thesis in the group of membrane trafficking led by professor Geert van den Bogaart PhD at the department of Tumor Immunology, within the Radboud Institute for Molecular Life Sciences (RIMLS). Currently, Maxim is a postdoctoral fellow at the department of Molecular Immunology and Microbiology at GBB, Groningen.
- Promotors: Prof. C.G. Figdor, Prof. G. van den Bogaart (RUG)