Center for Infectious Diseases Mycology course for technicians
13 January 2020 to 17 January 2020

This course is specially designed for lab technicians, but also other workers in lab diagnostics may wish to attend.

The Center of Expertise in Mycology of Radboud University Medical Center / Canisius Wilhelmina Hospital is organizing a hands-on course on fungal identification. This center is part of the Radboudumc Center for Infectious Diseases. This course is specially designed for lab technicians, but also other workers in lab diagnostics may wish to attend. Background of fungal classification will be explained in detail, with both theoretical and practical sessions. The relevant fungi will be shown, and brief introductions will be provided into other diagnostic techniques, such as PCR and Maldi-tof. A special session will be devoted to susceptibility testing. The course will be didactic in such a way that no special mycological background is required. The one-week course will terminate with an examination and certificate. Lectures will be given by Hein van der Lee, Sybren de Hoog and Paul Verweij.

Course fee is €1000.

As course materials, Larone’s Guide to Medically Important Fungi and the online version of the Atlas of Clinical Fungi will be used. Participants are requested to bring their own laptop and lab coat.


Venue

Radboud university medical center
Medical Microbiology, route 777
Geert Grootteplein- Zuid 10
6525 GA Nijmegen
The Netherlands
 
  • Day 1: Monday 13 January 2020

    Introduction to mycology
    Theory:      

    • Classification fungi, sporulation, determination fungi and used media, learn to work with Larone and the online database Atlas of Clinical Fungi. 

    Practice:    

    • Phenotypic identification of fungi, microscopic and macroscopic.

     

    Day 2: Tuesday January 14, 2020

    Dermatophytes
    Theory:     

    • Clinical appearance, taxonomy, problems of molecular identification, microscopy.


    Practice:    

    • Phenotypic identification of dermatophytes, microscopic and macroscopic.
    • ID of 5 unknown strains, self-learning.

     

    Day 3: Wednesday January 15, 2020

    Yeasts and MIC testing
    Theory:     

    • Mycoses by yeasts, including Candida, Cryptococcus and Malassezia.
    • Antifungal susceptibility testing.
    • Alternative techniques: 
    • Summary of molecular methods. Commercial kits, Dermagenius. MALDI-TOF.

    Practice:    

    • Identification of yeasts and MIC determinations.
    • ID of 2 unknown strains, self-learning.
    • Demonstration of rapid methods, MALDI.

     

    Day 4: Thursday January 16, 2020

    Opportunistic mycoses
    Theory:     

    • Aspergillosis, zygomycosis, Fusarium.

    Practice:    

    • Identification 25 opportunistic fungi.
    • Histology.

     

    Day 5: Friday January 17, 2020

    Subcutaneous and endemic mycoses
    Theory:     

    • Chromoblastomycosis, sporotrichosis, lobomycosis, rhinosporidiosis, blastomycosis, coccidioidomycosis, mycetoma, histoplasmosis.
    • Histology.

    Practice:   

    • Demonstration of 10 rare opportunistic fungi.


    Final examination