Regulation of epithelial calcium transportThis research group works on the regulation of epithelial calcium transport, with a fundamental focus on the electrophysiological analysis combined with the biochemistry of ion channels and cell signaling.
Characterization of the structure-function relationship of epithelial ion channels.
CharacterizationThrough uniquely synergizing cutting-edge (bio)chemical and biophysical technologies, we aim to deliver detailed insight into TRPV (vanilloid subfamily) channels and unravel structural domains essential for channel function. A high-resolution structure aids in unravelling the pathogenicity of specific disease variants and can provide detailed insight into the actions of pharmacological compounds, to ultimately support drug development programs.
Understanding the molecular mechanisms involved in renal calcium handling and kidney stone formation.
UnderstandingThe urinary calcium concentration is an important factor in kidney stone formation, and it is evident that avoidance of new crystal deposits should be the prime target site in order to prevent recurrence. Here, we focus on gaining fundamental knowledge on the related process of how perturbations in tubular calcium transport induce kidney stone formation. In addition, we aim to develop of a new molecular entity with a unique mode of action that might in the future be used for effective treatment of kidney stones.
Kidney organoids as tool to study electrolyte homeostasis
Kidney organoidsOrganoids constitute self-renewing 3D cultures that retain many structural and functional properties exhibited by the organ of origin. Kidney organoids may serve as disease model, provide new drug screening opportunities, and exhibit a high potential for treatment of renal disorders such as inherited electrolyte disorders. Therefore, it is essential to gain better understanding of electrolyte transport and its molecular regulation in kidney organoids. We are currently optimizing organoid culture and evaluating the transport of several electrolytes (Na+, Ca2+, Mg2+) in different types of kidney organoids (i.e. induced pluripotent stem cell (iPSC)-derived, adult stem cell-derived).