Generate lists of molecular machinesThis group aims to generate detailed parts lists of the molecular machines that are active in the cilia, and identify either their structural role in the cilium, or their role in the signaling pathways that are dependent on/directed by cilia.
- We employ next-generation sequencing techniques to identify pathogenic variations in ciliopathy patients
- Bioinformatic tools are used to organize the experimental proteomics results into relational networks. The functional features of these networks are analyzed by using (quantitative) proteomics and cell-based assays
- We use cultured ciliated cells as well as model organisms such as mice, zebrafish and Drosophila. Analysis of the complex interplay of these ciliary protein networks in health and disease will provide essential knowledge of the molecular basis of ciliopathies, which will pave the way for development of therapies