About our equipment

The technology center Flow cytometry holds nine flow cytometers (ranging from 4-colors up to 10-colors) and a flow sorter (18-color) that are available for research purposes. These are located within a ML-I/II biohazard room allowing analysis and sorting of genetically modified cells. A dedicated room is available for sorting viral-transduced and transfected cells with the required ML-I/II biosafety permit.

The research flow cytometers are supervised by senior specialists who train, advise and supervise internal and external personnel within the facility.
Research Facilities Flow cytometry

About our equipment

The technology center Flow cytometry holds nine flow cytometers (ranging from 4-colors up to 10-colors) and a flow sorter (18-color) that are available for research purposes.

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Using our equipment

Users who have operated flow cytometry equipment will, after evidence of proficiency, be allowed to use the equipment unassisted. Individual and group training in the use of our equipment is available to allow for unassisted use in the future.

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Using our equipment

Where

NIG Building M379 room number 4.148 (route number 475).

When

Mondays through Fridays from 8:00 to 18:00 hrs.

Reservations flow cytometry analysers

Click here to reserve via the booking platform.

Reservations for sorting

You can make a reservation request using your own Outlook agenda and invite the cell sorter.
  • In outlook select calendar (agenda) and then new (nieuw)
  • Select guide card invite attendees (deelnemers uitnodigen)  and send to (aan) Vz apparatuur Laboratoriumgeneeskunde LH flowcytometer Aria (VZ00329) and add this to required (vereist).
  • You can make this appointment. Select scheduling (planning). The bars indicate when the cell sorter is busy. Select the time you need to sort you samples.
  • Select appointment (afspraak). Mention your name and phone number under subject (onderwerp).
  • Finally, press send (verzenden).

Training

Contact Head Operator Rob Woestenenk at +31 (0)24 361 52 32 for instructions.


Beckman Coulter FC500 Flow cytometry analyzer

Designed for powerful performance and unmatched simplicity conducts 5-color analysis from either single 488nm or dual laser 635nm excitation. The FC500 can be used with a variety of fluorochromes to analyze five colors (GFP/FITC, PE, ECD, PC5 or APC (635nm) and PC7). Acquistion software is CXP and it functions with a carousel (32 tubes).

Example applications

  • Immunophenotyping (direct/ intracellular staining)
  • Viability (apoptosis)
  • Cell Counting
  • Nucleic Acid Analysis (Cell cycle analysis)
  • Platelet function analysis
  • Cell function analysis (ca efflux)
  • Cytokines (Cytokine bead array)


Beckman Coulter Gallios Flow cytometry analyzer

The 10 color Beckman Coulter Gallios is a research system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed datacollection.The system has 3 solid state lasers:
  1. 488nm excitation; FITC, PE, ECD, PC5, PC7
  2. 405nm excitation; Pacific Blue/BV421 and Krome Orange/BV510
  3. 642nm excitation; APC, APC-A700 and APC-A750

A selection from up to 62 parameters can be processed per analysis, at acquisition rates of 25,000 events-per-second. Acquistion software is Kaluza G (2014) and it functions with a carousel (32 tubes)

Examples application

  • Immunophenotyping (direct, intracellular, tetramer stainings)
  • Viability (apoptosis)
  • FRET Analysis (CFP YFP)
  • Nucleic Acid Analysis (cell cycle analysis)
  • Cell function analysis (ca efflux)
  • Combinatorial encoding MiHA-multimer assay


Beckman Coulter Cyan ADP Flow cytometry analyzer

CyAn ADP (three laser) is a 9-color flow cytometer.  It has 11 parameters (FSC, SCC, FL1-FL9) and is equipped with 488 nm, 405nm, and 642 nm solid-state lasers. The CyAn ADP has state-of-the-art optics, utilizes high performance digitization with pulse processing speed up to 70,000 events per second. The following colors can be measured:

  1. 488nm excitation; FITC, PE, ECD, PC5, PC7
  2. 405nm excitation; Pacific Blue and Pacific Orange
  3. 642nm excitation; APC and APC-Cy7

The Cyan is a flexible system other combinations are possible.  We have filter sets for Qdots / Brilliant Violets and Alexa 700. Acquistion software is Summit.

Example applications

  • Immunophenotyping (direct, intracellular, tetramer stainings)
  • Viability (apoptosis)
  • FRET Analysis (CFP YFP)
  • Nucleic Acid Analysis (Cell cycle analysis)
  • Cell function analysis (Ca efflux)
  • Combinatorial encoding MiHA-multimer assay (Qdots)


Becton Dickinson Aria Flow cytometry sorter

The high-speed sorting cytometer is equipped with 5 lasers, allowing excitation with UV (355nm), 405,  488, 561, 640 nm. There are 18 fluorescence detectors, allowing analysis of 18 colors and FSC and SSC. This provides a powerful and flexible analysis platform suitable for a wide range of research applications. Cell subsets identified using this powerful analysis capability can if required be sorted, using an electrostatic deflection mechanism.

Analysis/sort rates of over 15000 cells per second are possible, and sorted cells (4 populations at the same time) may be collected in tubes, onto slides, or into the wells of microplates. There is a sample collection temperature control option available.The instrument is fitted with a robotic arm allowing the programmed deposition of specified numbers of particular cell types, for example into each well of a 96 well plate. Sorting purity should be >99%.

The following colors can be measured

  • 355nm excitation (3 colors); Brilliant UV395, BUV496/Hoechst, Indo-1, BUV737
  • 488nm excitation (3 colors); FITC/GFP, PerCP, PerCP-Cy5.5
  • 405nm excitation (5 colors); CFP/Pacific Blue/Brilliant Violet 421 , Pacific/ Krome Orange/BV510, Qdot 605/ BV605, Qdot 705/BV711 and Qdot 800/BV786
  • 561nm excitation (4 colors); PE, ECD/mCherry, PECy5, and PECy7
  • 640nm excitation (3 colors); APC, Alexa 700 and APC-A750

Getting there

Entrance: Radboudumc main entrance (hoofdingang)
Route: 475, room number 4.148

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Getting there

Visiting address

Radboudumc main entrance
Geert Grooteplein Zuid 10
6525 GA Nijmegen

Directions

Go to Geert Grooteplein Zuid 10
Enter building at: Radboudumc main entrance (hoofdingang)
Follow route 475, room number 4.148

Radboudumc Technology Center Flow cytometry

At the Radboudumc extensive experience in flow cytometry is used in basic and translational research as well as clinical practice and clinical trials. We offer scientists advice and access to flow cytometry analyzers and sorters.

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