Vacancies 117601-PhD-candidate-Zebrafish-model-for-paragangliomas

About the position

  • 36 hours per week
  • Temporary
  • 3 years
  • Salary scale 10A
  • Fulltime: min € 2570 - max € 3271 gross per month
  • Apply before 9 June 2022

#weareradboudumc, what about you?


Job description

Pheochromocytomas and paragangliomas (PPGLs) are rare neuroendocrine tumors originating from the adrenal medulla or sympathetic ganglia, respectively. Currently, there is no effective treatment for patients with metastatic or inoperable PPGLs. Patients with mutations in the β-subunit of the...

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Job description

Pheochromocytomas and paragangliomas (PPGLs) are rare neuroendocrine tumors originating from the adrenal medulla or sympathetic ganglia, respectively.  Currently, there is no effective treatment for patients with metastatic or inoperable PPGLs. Patients with mutations in the β-subunit of the succinate dehydrogenase (SDHB) have the highest risk to develop incurable malignant PPGLs.

Therapy development is severely hampered by the limited availability of suitable model systems. The  succinate dehydrogenase (SDH) complex has a major role in tumorigenesis, which we try to further unravel using zebrafish as animal model organism. We generated a first vertebrate animal model that mimics the metabolic effects of SDHB-associated PPLGs which allows us for the first time to study the metabolic effects of the lack of sdhb in vivo. This larval model will we used as powerful drug screening tool to provide valuable insights into pathomechanisms. In parallel, the adult fish will be used as functional tumor model. For this, a comprehensive toolbox for tumor detection and follow tumor growth in our sdhbrmc200 zebrafish model is ready for use.

In this project, strategies will be applied to accelerate tumor development in adult zebrafish to unravel underlying pathogenic mechanisms and to evaluate therapeutic strategies  in vivo. You will develop and apply new tools to study and accelerate the process of tumorigenesis in adult zebrafish. In parallel, the obtained data will be compared and validated in available human samples. The role of especially the immune system, epigenetics, oxidative stress and DNA repair will be studied in detail. Ultimately, you will explore the potential of new therapeutic targets to treat malignant SDHB-associated PPGLs.

Tasks and responsibilities

  • Characterization of inter-renal tumor formation in systemic and tissue-specific sdhb mutant zebrafish.
  • Application and development of aggravation strategies to induce tumor formation in adult zebrafish.
  • Investigating the role of oxidative stress, DNA repair, epigenetics and the immune system in tumorigenesis.
  • Identification of novel therapeutic targets and drugs.
  • Comparison and validation of zebrafish data with available human samples.
  • Generate and critically analyse the data in order to contribute to scientific publications and presents findings at scientific meetings.
  • Work in a highly international research group with fellows from many countries.
  • Supervise MSc and/or BSc students.
  • Write and defend a PhD thesis within three years of starting the project.
  • Participation in the graduate school of Radboudumc.


 


Department

The position is available at the Department of Internal Medicine in close collaboration with the Department of Ecology and Physiology at the Radboud Institute Biological and Environmental Sciences ( RIBES) . The laboratory of Experimental Internal Medicine is world renowned for its translational...

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Department

The position is available at the Department of Internal Medicine in close collaboration with the Department of Ecology and Physiology at the Radboud Institute  Biological and Environmental Sciences (RIBES). The laboratory of Experimental Internal Medicine is world renowned for its translational research on inflammation/innate immunity and harbors approximately 50 PhD candidates, 10 postdocs and 10 technicians.

You will join the group of Dr. Margo Dona, investigating new treatment possibilities for a rare type of neuroendocrine cancer called pheochromocytomas and paragangliomas. This fundamental research will be performed in close collaboration with clinical expertise within the Radboudumc Centre of Expertise for Adrenal Disorders, headed by Prof. Dr. Henri Timmers.  

Research Institutes
At the moment there are more than 1,300 PhD candidates at our medical hospital. This number includes PhD candidates on our pay roll as well as external candidates (those employed somewhere else but researching on our premises).

  • Radboud Institute for Health Sciences: ± 700
  • Radboud Institute for Molecular Life Sciences: ± 400
  • Donders Center for Medical Neurosciences: ± 200
Read what it is like to do a PhD at the Radboud University Medical Center.

Radboudumc
Radboud university medical center is a university medical center for patient care, scientific research, and education in Nijmegen. Radboud university medical center strives to be at the forefront of shaping the healthcare of the future. We do this in a person-centered and innovative way, and in close collaboration with our network. We want to have a significant impact on healthcare. We want to improve with each passing day, continuously working towards better healthcare, research, and education. And gaining a better understanding of how diseases arise and how we can prevent, treat, and cure them, day in and day out. This way, every patient always receives the best healthcare, now and in the future. Because that is why we do what we do.

Read more about our strategy and what working at Radboud university medical center means. Our colleagues would be happy to tell you about it. #weareradboudumc


Profile

You have as structured working style and a strong motivation, flexibility, and willingness to learn. You have a critical scientific attitude and are able to work both independently as well as in a group. Furthermore: You have a MSc degree in Biomedical Sciences or Molecular Life Sciences or a...

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Profile

You have as structured working style and a strong motivation, flexibility, and willingness to learn. You  have a critical scientific attitude and are able to work both independently as well as in a group. Furthermore: 

  • You have a MSc degree in Biomedical Sciences or Molecular Life Sciences or a related field.
  • Experience with molecular and cell biology is considered a strong plus.
  • Article 9 of the Dutch Law on Animal Experimentation is considered a strong plus.
  • Experience with in vivo modeling using zebrafish is considered a plus.
  • Experience with genome editing e.g. CRISPR-cas9 and cloning is considered a plus.
  • Experience with bio-informatics is considered a plus.
  • Excellent communication skills; oral and in writing.

Let's meet


Terms of employment

Working at Radboud university medical center means that you are ahead of the curve and working together on the healthcare of the future. And there is more. Our secondary terms of employment are impressive. These are fully tailored to you thanks to our Employment Conditions Selection Model. At...

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Terms of employment

Working at Radboud university medical center means that you are ahead of the curve and working together on the healthcare of the future. And there is more. Our secondary terms of employment are impressive. These are fully tailored to you thanks to our Employment Conditions Selection Model. At Radboud university medical center, you will be given trust, and you will take the responsibility to handle everything together. We provide annual courses, both professional and personal.

  • In addition to your monthly salary and an annual vacation allowance of 8%, you will receive an end-of-year bonus of 8.3%.
  • If you work irregular hours, you will receive an allowance.
  • As a full-time employee (36 hours per week), you are entitled to approximately 168 vacation hours (over 23 days) per year.
  • Radboud university medical center pays 70% of the pension premium. You pay the rest of the premium with your gross salary.
  • You get a discount on health insurance as well: you can take advantage of two group health insurance plans. UMC Zorgverzekering and CZ collectief.
 
In addition to our terms of employment, we also offer employees various other attractive facilities, such as childcare and sports facilities. Want to learn more? Take a look at the Cao UMC.


Application procedure

Applicants should include a letter of intent outlining special interest in the position, related qualifications, experience and career goals, a curriculum vitae, including a list of grades of your BSc MSc components and names and addresses of professional references in the application. We would...

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Application procedure

Applicants should include a letter of intent outlining special interest in the position, related qualifications, experience and career goals, a curriculum vitae, including a list of grades of your BSc MSc components and names and addresses of professional references in the application. We would like to receive your application before 9 June 2022. We will then contact you shortly.

What else can you expect?

When you begin employment, we will request a certificate of conduct (VOG) and, depending on the position, you may be screened. You do not need to do anything for this; we will inform you later. 
 
Radboud university medical center is an employer that welcomes everyone. Every colleague brings valuable experience, expertise, and creativity. We believe in the benefits of a diverse and inclusive organization. #weareradboudumc

Read more about our application procedure or take a look at the frequently asked questions
 
We are recruiting for this vacancy ourselves. We only accept applications directly from the applicants themselves, but intermediaries are welcome to share our vacancies in their networks.​


Contact

All additional information about the vacancy can be obtained from Dr. Margo Dona , Postdoc via +31 6 12 82 69 01 or from Prof.Dr. Henri Timmers , Professor of endocrinology at +31 24 361 77 37. Use the Apply button to submit your application.

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Contact

All additional information about the vacancy can be obtained from Dr. Margo Dona, Postdoc via +31 6 12 82 69 01 or from Prof.Dr. Henri Timmers, Professor of endocrinology at +31 24 361 77 37. Use the Apply button to submit your application.

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