- 36 hours a week
- 4 years
- Scale 10A: max € 41835 gross per year at full employment (incl. vacation bonus and end of year payments)
Job descriptionCentral areolar choroidal dystrophy (CACD) is a retinal disorder characterized by progressive photoreceptor degeneration and central vision loss, usually starting in young adulthood. CACD is most commonly caused by heterozygous mutations in the PRPH2 gene, which encodes a tetraspanin protein essential for the formation of outer segment discs in the photoreceptor cells. Yet, to date, the exact molecular mechanism by which PRPH2 mutations exert their pathophysiology is still unknown, as is the understanding why in some families, individuals harboring disease-causing PRPH2 mutations are not affected. In addition, an effective treatment for PRPH2-associated CACD is still lacking.
In this project, we aim to understand the relationship between PRPH2 mutations and the onset of CACD, and to develop allele-specific therapeutic strategies. For this, we will use state-of-the-art sequencing methods and molecular techniques, and a variety of cellular models including patient-derived fibroblast cells, induced pluripotent stem cells, and retinal organoids.
Tasks and responsibilities
- Plan and perform scientific in vitro and in vivo experiments in an independent manner;
- Critically analyze data;
- Take a leading role in writing manuscripts;
- Present data at local, national and international scientific meetings;
- Supervise MSc and/or BSc students;
- Write and defend a PhD thesis within four years of starting the project.
ProfileCandidates must have completed a MSc degree in Molecular Life Sciences or a related field. They need to have a solid theoretical background in molecular and cell biology, as well as hands-on experience with standard molecular techniques such as PCR, cloning, RT-PCR, western blotting and cell culture. Experience with next-generation sequencing technologies, stem cell technology and/or molecular therapy is considered a strong plus.
Candidates should have a strong ambition to succeed in science, be result-oriented, and have excellent communication skills (both orally and in writing). They should possess a critical scientific attitude and should be able to work both independently as well as in a team.
OrganizationThe PhD candidate will be positioned in two groups, i.e. within the “Molecular Therapies for Inherited Eye Diseases” group led by Dr. Rob Collin, and the “Molecular Ophthalmology” group led by Prof. den Hollander. Dr. Collins lab aims to develop various therapeutic interventions for inherited retinal diseases, with a strong focus on bringing new promising therapies to the clinic. Prof. den Hollanders group focuses on the exact mechanisms how certain genetic variants contribute to disease onset and severity.
Both the Department of Human Genetics and the Department of Ophthalmology at the Radboudumc in Nijmegen are internationally renowned for their research, molecular and clinical diagnostics of inherited retinal disease. The Departments offer an exciting working environment with enthusiastic people from many parts of the world, providing ample critical mass and a stimulating, collaborative atmosphere for high quality research.
Radboudumc strives to be a leading developer of sustainable, innovative and affordable healthcare to improve the health and wellbeing of people and society in the Netherlands and beyond. This is the core of our mission: To have a significant impact on healthcare. To get a better picture of what this entails, check out our strategy.
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Employment conditionsUpon commencement of employment we require a certificate of conduct (Verklaring Omtrent het Gedrag, VOG) and there will be a screening based on the provided CV. Radboud university medical center’s HR Department will apply for this certificate on your behalf.
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Comments and contact informationAnticipated starting date: 1st of March 2019
Applications should be accompanied by a motivation letter and CV, including a list of grades of your BSc MSc components, both in English, as well as names and contact information of two references.
All additional information about the vacancy can be obtained from Dr. Rob Collin, Associate Professor, via +31 (024) 361 37 50. Use the Apply button to submit your application.
This vacancy is open until filled.
Recruitment agencies are asked not to respond to this job posting.
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