- 36 hours a week
- 4 years
- Date of publication: 21 December 2018
- Deadline: 4 February 2019
- Scale 10A: max € 42220 gross per year at full employment (incl. vacation bonus and end of year payments)
- First interview scheduled: week 7
Job descriptionCiliopathies are a group of severe, genetically heterogeneous hereditary disorders that are caused by a dysfunctional cilium, an organelle that acts as the cell’s antenna and signaling hub. The disorders are characterized by variable, overlapping defects in several organs and tissues, often including the brain, the retina, and the kidneys. Recent studies in our lab have unveiled intriguing mechanistic insights into the disrupted processes that may underlie the organ-specific defects.
In this project, we aim to scrutinize these processes, as well as identify therapeutic compounds that could antagonize the disruptive effects. For this, we will use state-of-the-art molecular techniques, including CRISPR/Cas9-based genome editing, super-resolution microscopy, affinity and proximity proteomics, and a variety of cellular models including induced ciliated mammalian cells, pluripotent stem cells, retinal organoids, and brain organoids.
Tasks and responsibilities
- Plan and perform scientific in vitro and in vivo experiments in an independent manner;
- Critically analyze data;
- Take a leading role in writing manuscripts;
- Present data at local, national and international scientific meetings;
- Supervise MSc and/or BSc students.
ProfileCandidates must have completed a MSc degree in Molecular Life Sciences or a related field. They need to have a solid theoretical background in and affinity with cell biology and biochemistry, as well as hands-on experience with standard molecular techniques such as PCR, cloning, RT-PCR, western blotting, immunocytochemistry, and cell culture. Experience with (induced pluripotent) stem cell technology, CRISPR/Cas9 based genome editing, and/or molecular therapy is considered a strong plus.
Candidates should have a strong ambition to succeed in science, be result-oriented, and have strong communication skills (both orally and in writing). They should possess a critical scientific attitude and should be able to work both independently as well as in a team.
OrganizationThe PhD candidate will be part of the ‘Molecular Biology of Ciliopathies’ group, led by Prof. Ronald Roepman, that focuses on deciphering the molecular mechanisms that underlie ciliary disorders. Part of the work will be performed in the group of Dr. Nael Nadif Kasri, who focuses on the synaptic basis of network dysfunction in neurodevelopmental disorders. The project is also a close collaboration with the group of Prof. Dr. Helen May-Simera in Mainz, Germany, and an exchange visit to her lab will be part of this PhD project.
The department of Human Genetics of the Radboudumc in Nijmegen is internationally renowned for its research and diagnostics on a variety of genetic and genomic disorders. The Department offers an exciting international working environment with enthusiastic people from many parts of the world, providing ample critical mass and a stimulating, collaborative atmosphere for high quality research.
Radboudumc strives to be a leading developer of sustainable, innovative and affordable healthcare to improve the health and wellbeing of people and society in the Netherlands and beyond. This is the core of our mission: To have a significant impact on healthcare. To get a better picture of what this entails, check out our strategy.
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Employment conditionsUpon commencement of employment we require a certificate of conduct (Verklaring Omtrent het Gedrag, VOG) and there will be, depending on the type of job, a screening based on the provided cv. Radboud university medical center’s HR Department will apply for this certificate on your behalf.
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Comments and contact informationAnticipated start date: as soon as possible, not later than May 1st, 2019.
Applications should be accompanied by a motivation letter and CV, both in English, as well as names and contact information of two references.
All additional information about the vacancy can be obtained from Prof. Dr. Ir. Ronald Roepman, principal investigator, chair Molecular Biology of Ciliopathies via +31 (0)24 361 08 68. Use the Apply button to submit your application.
Please apply before 4 February.
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