Vacancies 96781-Research-technician-Sugar-metabolism-in-stem-cell-models

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About the position

  • 36 hours per week
  • Temporary
  • maximum of 5 years
  • Salary scale 7
  • min € 2422 - max € 3280 gross per month for full-time employment
  • Deadline: 10 May 2021

#weareradboudumc, what about you?


Job description

We are looking for an enthusiastic and ambitious research technician to help studying the metabolism of sialic acid in stem cell models of muscle and brain.

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Job description

We are looking for an enthusiastic and ambitious research technician to help studying the metabolism of sialic acid in stem cell models of muscle and brain.
Sugar metabolism delivers the building blocks for protein glycosylation. This essential process is believed to be similar in different body organs. Our research on patients with genetic defects in protein N-glycosylation has revealed novel and unexpected evidence for tissue-specific regulatory mechanisms in muscle and brain. In this recently granted prestigious Vici project, we will zoom in on the important sialic acid pathway to uncover regulatory mechanisms in muscle and brain.

We will employ engineered neurons and muscle cells as models, derived from patient’s induced pluripotent stem cells and via genome editing. We will integrate novel metabolic flux methods for steady state and dynamic analysis of sugar metabolism by mass spectrometry. In addition to identifying novel regulatory mechanisms, we aim to identify novel therapeutic strategies by use of synthetic sugars and molecular genetic approaches. As research technician, you will support the work of two PhD students (see vacancy) and more experienced researchers on this project with generation of stem cell models and genome editing.

Tasks and responsibilities

  • Support in generation of stem cell models from patient-derived induced pluripotent stem cells (iPSCs).
  • Genome editing of iPSCs.
  • Metabolite extraction and analyses.
  • General organization of the research group.


Department

The project is embedded in the Radboudumc at the interface of the departments of Neurology , Human Genetics , and the Translational Metabolic Laboratory . Research in these departments is focused on Neurogenetic diseases, aiming for improved diagnosis, better understanding of neuropathophysiology...

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Department

The project is embedded in the Radboudumc at the interface of the departments of Neurology, Human Genetics, and the Translational Metabolic Laboratory. Research in these departments is focused on Neurogenetic diseases, aiming for improved diagnosis, better understanding of neuropathophysiology by use of model systems and testing novel therapeutic strategies. The current position is shared between the Lefeber group on Glycosylation Disorders and the Nadif Kasri group on neuronal model systems. The combined infrastructure is highly innovative including the Radboudumc Technology Center for mass spectrometry (proteomics, glycomics and (sugar)metabolomics) with a wide range of analytical equipment and mass spectrometers, the department of Human Genetics with all modern genomics tools, including next-generation sequencing, CRISPR/Cas9 genome editing, and the Radboudumc stem cell facility. The research of both groups is embedded in the Donders Institute for Medical Neuroscience which harbors a range of top researchers in the field of neurogenetic disease.

Radboudumc
Radboud university medical center is a university medical center for patient care, scientific research, and education in Nijmegen. Radboud university medical center strives to be at the forefront of shaping the healthcare of the future. We do this in a person-centered and innovative way, and in close collaboration with our network. We want to have a significant impact on healthcare. We want to improve with each passing day, continuously working towards better healthcare, research, and education. And gaining a better understanding of how diseases arise and how we can prevent, treat, and cure them, day in and day out. This way, every patient always receives the best healthcare, now and in the future. Because that is why we do what we do.

Read more about our strategy and what working at Radboud university medical center means. Our colleagues would be happy to tell you about it. #weareradboudumc.


Profile

Are you an enthusiastic research technician with a positive attitude? Do you have a strong interest in academic research? Are you highly motivated and flexible to work in an interdisciplinary environment? And do you recognize yourself in the following profile? Then we are looking for you! Degree...

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Profile

Are you an enthusiastic research technician with a positive attitude? Do you have a strong interest in academic research? Are you highly motivated and flexible to work in an interdisciplinary environment? And do you recognize yourself in the following profile? Then we are looking for you!

  • Degree as research technician with background in one of the molecular life sciences.
  • Experience with induced pluripotent stem cell derived models and genome editing.
  • Excellent organizational and communication skills.

Let's meet


Terms of employment

Working at Radboud university medical center means that you are ahead of the curve and working together on the healthcare of the future. But there is more. Our secondary terms of employment are impressive. These are fully tailored to you thanks to our Employment Conditions Selection Model. At...

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Terms of employment

Working at Radboud university medical center means that you are ahead of the curve and working together on the healthcare of the future. But there is more. Our secondary terms of employment are impressive. These are fully tailored to you thanks to our Employment Conditions Selection Model. At Radboud university medical center, you will be given trust, and you will take the responsibility to handle everything together. We provide annual courses, both professional and personal.

  • In addition to your monthly salary and an annual vacation allowance of 8%, you will receive an end-of-year bonus of 8.3%.
  • If you work irregular hours, you will receive an allowance (47% or 72%) for this.
  • As a full-time employee, you are entitled to approximately 168 vacation hours (over 23 days) per year.
  • Radboud university medical center pays 70% of the pension premium. You pay the rest of the premium with your gross salary.
  • You get a discount on health insurance as well: you can take advantage of two group health insurance plans. UMC Zorgverzekering and CZ collectief.
The intended start date is 1 July 2021. 

In addition to our terms of employment, we also offer employees various other attractive facilities, such as childcare and sports facilities. Want to learn more? Take a look at the CAO UMC.


Apply at Radboud university medical center

  • What can you expect when you apply for a job at Radboudumc?


Contact

All additional information about the vacancy can be obtained from Prof. Dr. Dirk Lefeber , Glycosylation Disorders in Neurology, via +31(0)24 361 45 67. Use the Apply button to submit your application.

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Contact

All additional information about the vacancy can be obtained from Prof. Dr. Dirk Lefeber, Glycosylation Disorders in Neurology, via +31(0)24 361 45 67. Use the Apply button to submit your application.

If you are motivated to join our team, please send your CV, names and addresses of two professional references and a letter describing your motivation for this project. We would like to receive your application before 10 May 2021.

What else can you expect?
When you join us, we require a certificate of conduct (Verklaring Omtrent het Gedrag, VOG) and there will be, depending on the type of job, a screening based on the provided cv. You do not have to do anything for this; we will tell you more later. Read more what our International Office can do for you when moving to the Netherlands, about the application process or view our frequently asked questions.

Recruitment agencies are asked not to respond to this job posting.

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