Cellular activities are dictated by proteins. Deciphering how proteins interact in a healthy or diseased state is a key area of interest in biology. Eventually, this will facilitate the development of drugs for many diseases including cancer, which is ultimately the result of misregulated protein homeostasis.In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular protein-protein interactions. Unfortunately, AP-MS experiments typically require large amounts (millions of cells) of input material. For this reason, AP-MS studies are mostly restricted to cancer cell lines that can be grown in large quantities in vitro. Cristina Furlan and René Dirks from the groups of Michiel Vermeulen and Hendrik Marks, research theme Cancer development and immune defense, teamed up to address these limitations by developing a low input AP-MS workflow, called on-chip AP-MS, which can be used to perform AP-MS experiments using a few micrograms of lysate from a few thousand cells. This was achieved by implementing an AP-MS method in a automated, microfluidic device from the company Fluidigm using nanoliter volumes.
With the development of on-chip AP-MS, clinical samples, rare cell types and organoids, which are difficult to obtain in large quantities, can now be investigated using interaction proteomics studies. The field of mass spectrometry-based interaction proteomics is thus taking an important next step towards applications in biology and medicine which thus far, due to technical constraints, have remained elusive. This study was recently published in Nature Communications.
Related news items
Radboud Bioinformatics Xmas event 16 December21 October 2019
The CMBI is organizing the event of the year for all bioinformaticians throughout Radboud University and Radboudumc to give you the opportunity to bring forward the biggest challenges in your research to expert bioinformaticians and pave the way for real solutions.read more
Merel Ritskes-Hoitinga has been appointed honorary Skou professor at Aarhus University17 October 2019
Merel Ritskes-Hoitinga has been appointed honorary Skou Professor at the Dept. of Clinical Medicine, at Aarhus University. The aim is to introduce new perspectives to teaching, strengthen relations with leading research units abroad and establish joint research projects and funding applications.read more
A personal touch of Maroeska te Loo15 October 2019
In order to promote interaction amongst colleagues within RIMLS, we have a ‘personal touch’ series setting employees in the spotlight. A light-hearted manner to learn about the colleagues you know and those you don’t. This week: Maroeska te Loo.read more
KWF Roadshow 11 November 201910 October 2019
In which way(s) can KWF provide optimal support to oncological research and care? How can we maximize impact on our investments? These questions are pivotal in Ambition 2030: the vision that KWF developed in close cooperation with stakeholders in the oncological field.read more