4 April 2019

Cellular activities are dictated by proteins. Deciphering how proteins interact in a healthy or diseased state is a key area of interest in biology. Eventually, this will facilitate the development of drugs for many diseases including cancer, which is ultimately the result of misregulated protein homeostasis.

In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular protein-protein interactions. Unfortunately, AP-MS experiments typically require large amounts (millions of cells) of input material. For this reason, AP-MS studies are mostly restricted to cancer cell lines that can be grown in large quantities in vitro. Cristina Furlan and René Dirks from the groups of Michiel Vermeulen and Hendrik Marks, research theme Cancer development and immune defense, teamed up to address these limitations by developing a low input AP-MS workflow, called on-chip AP-MS, which can be used to perform AP-MS experiments using a few micrograms of lysate from a few thousand cells. This was achieved by implementing an AP-MS method in a automated, microfluidic device from the company Fluidigm using nanoliter volumes.

With the development of on-chip AP-MS, clinical samples, rare cell types and organoids, which are difficult to obtain in large quantities, can now be investigated using interaction proteomics studies. The field of mass spectrometry-based interaction proteomics is thus taking an important next step towards applications in biology and medicine which thus far, due to technical constraints, have remained elusive. This study was recently published in Nature Communications.
 

Related news items


Rob ter Horst in Dutch television broadcast Brandpunt Plus

21 May 2019

In a Dutch television broadcast, called Brandpunt Plus, Rob ter Horst, PhD candidate, theme Infectious diseases and global health, explains how he records data about himself and what he hopes to find out about himself.

read more

How do we keep new technology affordable to improve the lives of people?

16 May 2019

Improving healthcare by means of new technological developments? We need to change the entire approach for evaluating the true impact , says Maroeska Rovers. By bringing together all stakeholders from the beginning, millions of euros can be saved.

read more

RIMLSpeaks Challenge for change

15 May 2019

The third edition of RIMLSpeaks entitled “Challenge for change" was organized for all our (j)PI’s in Huize Heyendael.

read more

Join the Young Academy of Europe

15 May 2019

The YAE is a pan-European initiative of outstanding young researchers, from all fields, for networking, scientific exchange and science policy.

read more

Dutch Kidney Foundation Innovation grant for Tom Nijenhuis

14 May 2019

Tom Nijenhuis, theme Renal disorders, received this grant for his research project “Salt supplementation in Gitelman syndrome: A simple intervention using an innovative N-of-1 trial approach”.

read more

Algorithm calculates the presence and aggressiveness of prostate tumors

14 May 2019

A computer program that analyzes MRI images can reliably map the presence and even the aggressiveness of a prostate tumor. This is what Radboudumc researchers and international colleagues have published in the scientific journal Investigative Radiology.

read more