6 May 2021

Together with Fluidigm, a US-based company focusing on microfluidics, the team of Hendrik Marks publishes in Genome Research the development of a powerful plug and play ChIP-seq platform for minute amount of cells, such as embryonic specimens or small biopsies.

Epigenetic profiling by chromatin immunoprecipitation followed by sequencing (ChIP-seq) has become a powerful tool for genome-wide identification of regulatory elements, for defining transcriptional regulatory networks, and for screening for biomarkers. However, the ChIP-seq protocol for low-input samples is laborious and time-consuming and suffers from experimental variation, resulting in poor reproducibility and low throughput.

To enable standardized, automated ChIP-seq profiling of low-input samples, we constructed microfluidic PDMS-based plates capable of performing 24 sensitive ChIP reactions within 30 min of hands-on time and 4.5 h of machine-running time. These disposable plates can be conveniently loaded into a widely available controller for pneumatics and thermocycling. In light of the plug and play (PnP) ChIP plates and workflow, we named our procedure PnP-ChIP-seq. PnP-ChIP-seq allows nonexpert laboratories worldwide to conveniently run robust, standardized ChIP-seq, whereas its high throughput, consistency, and sensitivity pave the way toward large-scale profiling of precious sample types such as rare subpopulations of cells or biopsies.

Hendrik Marks and his team are RIMLS researchers who work at the Faculty of Science.

read more

Related news items


Radboud university medical center and Astherna join forces for new therapy for Stargardt disease

17 June 2021

RNA therapy for patients with a severe inherited retinal disease

read more

The international dataset for colorectal local excisions is published

16 June 2021

Iris Nagtegaal and colleagues presented their first paper on the colorectal cancer dataset and published this in the journal Gastroenterology.

read more

Neuraminidase and SIGLEC15 modulate the host defense against pulmonary aspergillosis

15 June 2021

Intan Dewi and Frank van de Veerdonk published in Cell Reports Medicine that Neuraminidase and SIGLEC15 modulate the host defense against pulmonary aspergillosis.

read more

ZonMw Open Competition Grants for several Radboud researchers

15 June 2021

Four teams including researchers from Radboudumc and Radboud University receive a ZonMw Open Competion grant. Each proposal has a maximum budget of 750K euros.

read more

ZonMw Open Competition Grant for Annemiek van Spriel and Piet Gros

15 June 2021

Annemiek van Spriel, theme cancer development and immune defense, together with Piet Gros, Dept. of Chemistry, Utrecht University obtained a ZonMw Open Competition Grant to investigate IL-6 receptor structure and signalling in tumor cells.

read more