Radboud Institute for Molecular Life Sciences Organization Laboratory Support & Media preparation

About LEM

LEM is responsible for the general supply of (specialized) lab media as well as for the collection and autoclaving of GGO waste and cleaning/sterilization of glassware. Read more about our services and feel free to get in touch with us for general or specific enquires, comments and suggestions.

Contact

Thea Chris

+31 (0)24 361 4257

Contact


Laboratory support Services

Glassware

GlasswareClean, sterilized glassware can be found on each floor in the cabinets located in the corridors alongside the laboratories. Glassware is  sterilized for 6 hours at 180 degrees Celsius ensuring complete cleanliness for laboratory work. Our autoclaves are regularly inspected to ensure they comply with the most up-to-date regulations. Dirty glassware is picked up by the LEM on a daily basis and returned to the storage cabinets.
 
Used glassware should be placed in the green transport containers located under the sinks in each laboratory and in the kitchens. Please rinse glassware and remove any stickers, parafilm or pen markings before placing in the containers. Avoid damage to glassware by not over-filling containers. Empty containers can be found in the kitchens.

Solutions and consumables

The LEM will autoclave laboratory solutions (e.g  MilliQ, PBS, medium and buffers) and consumables (e.g. disposable tips and Eppendorf tubes). Liquids should be placed in appropriate bottles or conical (Erlenmeyer) flasks. Solid material should be placed in an appropriate box with lid or bottle. Old autoclave tape and stickers should be removed. Never screw bottles shut; screw caps should be loosely fitted.
 
Solutions and consumables to be autoclaved should be placed in the kitchen on your own floor in the dedicated wire baskets provided. Bottles, flasks and boxes should have autoclave tape clearly labeled with your name, lab-nummer and floor. Material in the kitchen before 11:30am will be usually returned autoclaved the same day but always within 24 hours. For urgent material delivered after 11:30 please contact one of the LEM employees.

Laboratory coats

Dirty coats that need to be washed and that are not included in the Radboudumc rotation system can be brought to the LEM on Monday mornings. We will make sure that they are taken to the laundry. They are usually returned to us within 1 week. We will bring them back to your department.


GMO waste Services

More information: link



Standard media available Services

The LEM makes a range of media that can be found in the cabinets alongside the laboratories on each floor. Please get in touch with a LEM staff member for special requests.
 
NB: All media is made using Demi water with the exception of PBS 10X, TAE 50X and TBE 10X that are made using MilliQ water
 

Tris-Glycine (1000 ml Schott bottles) Demi water 

Litre

Tris

Glycine

1

30 gr

144 gr

 

TBE 10x (1000 ml Schott bottles) MilliQ water (Ph 8.3)

Litre

Tris Base

Boorzuur

EDTA

1

109 gr

60 gr

4,65 gr

 

Tris-HCL 0,5 M pH 6,8 (100 ml Schott bottles) MilliQ water

Litre

TRIS

1

60,55 gr

 

Tris-HCL1,5 M pH 8,8 (250 ml Schott bottles) MilliQ water

Litre

Tris

1

181,65 gr

 

TAE (50x, 1000 ml Schott bottles) MilliQ water

Litre

Tris Base

ACETIC ACID
(glacial)

500 mM EDTA
(pH 8,0)

1

242 gr

57.1 ml

100 ml

 

10x PBS (1000 ml Schott bottles, pH 6.5 - 6.8) MilliQ water *
NB: To dilute use Demi water NOT MilliQ

Litre

NaCl

KCl

Na2HPO4.H20

KH2PO4

1

80 gr

2 gr

13,8 gr

2,6 gr

 

1x PBS (1000 ml Schott bottles, pH 7.2 - 7.4) MilliQ water *
NB: To dilute use Demi water NOT Milliq 

Litre

NaCl

KCI

Na2HPO4

KH2PO4

1

8 gr

0,2 gr

1,38 gr

0,26 gr

 

10% SDS stock (100 ml Schott bottles) MilliQ water

Litre

SDS

1

100 gr. UltraPure (TM) sodium Dodecyl Sulfate (SDS)

 

 

L-B Agar (500 ml conical flasks) Demi water *

Litre

PEPTON

YEAST

NaCl

Agar

1

10 gr

5 gr

8 gr

15 gr

 

L-B Medium (100 ml & 250 ml Schott bottles) Demi water *

Litre

PEPTON

YEAST

NaCl

1

10 gr

5 gr

8 gr

 

2x TY medium (250 ml Schott bottles) Demi water *

Litre

PEPTON

YEAST

NaCl

1

16 gr

10 gr

5 gr

  * Autoclaved



Cryo storage facility Services

Liquid Nitrogen Level Control System (CRAS)

The M505CE is a Microprocessor based Liquid Nitrogen Level Control System with the following features and functions:
  • Level monitoring and automatic filling of refrigerators.
  • Level display (High/Normal/Low).
  • Temperature monitoring and display.
  • Temperature Control
  • Generation of local and remote alarms for:
    - High Temperature
    - High or Low liquid levels
    - Sensor faults
    - Failure of liquid nitrogen supply
    - Lid left open
In case off an alarm there will be automatically an SMS message to Ronald Scheffer, Martijn Verdoes or Jona Walk. There is no need to replay.

Contact persons Liquid Nitrogen Facility in case of ALARM

 

1. Ronald Scheffer

 Ronald.Scheffer@radboudumc.nl

  +31 (0)24 361 42 57

2. Martijn Verdoes

 Martijn.verdoes@radboudumc.nl

  +31 (0)24 361 05 57

3. Jona Walk

 Jona.walk@radboudumc.nl

 +31 (0)24 361 36 63

Contact persons Liquid Nitrogen Facility in case of management issues

 

1. Ben Joosten

 ben.joosten@radboudumc.nl

  +31 (0)24 361 46 73

2. Dov Ballak

 dov.ballak@radboudumc.nl

 +31 (0)24 369 36 52

3. Bert Gerrits en bedrijf techniek

 

 +31(0)24 361 33 83 /
 +31(0)24 361 80 00



Freezer storage facility Services

As well as housing the technical facilities for the RIMLS research building, the 4th floor accommodates serveral facilities.
  • A back-up/emergency  -80°C freezer is also available.
    This freezer may be reserved (max 1 day). For more info contact postbusrimls.lem@radboudumc.nl tel. 14257.



Las 4.000 Odyssey Services

ImageQuant LAS 4000

ImageQuant LAS 4000 is a digital imaging system for sensitive, quantitative imaging of gels and blots, by chemiluminescence and fluorescence. A flexible combination of light sources and filters can be incorporated into the imager according to your needs.
 
ImageQuant LAS 4000 is designed to quantitate the highly sensitive detection of proteins using Amersham ECL Western blotting reagents. A free combination of light sources and filters can be incorporated into the imager according to your needs.
 
The system consists of an intelligent dark box, camera, lens, and automated four-position filter wheel with a standard filter for EtBr and open positions for the filters delivered with the optional fluorescent light sources. Focusing, filters, illuminators, and exposure time are remotely controlled by a computer. Simple operation allows adjustment without having to open the cabinet. The intelligent light box provides automation and user safety. UV light sources are housed inside the cabinet, and can only be switched on when the door is closed.
 
A bright, wide aperture FUJINON™ F0.85 lens, specially developed for chemiluminescence imaging, projects sharp images onto a patterned CCD chip. By diagonally aligning octogonal pixels, the gap between pixels in the horizontal and vertical planes is reduced. This pattern allows an effective image resolution of 6.3 Mpixels.
 
The user-friendly control software has several capture modes for achieving optimal sensitivity and dynamic range including increment, repetition, and program modes. For image analysis, advanced and flexible ImageQuant TL software is available separately and comprises a dedicated suite of analysis applications to provide high levels of automation and accuracy in analysis of gels, blots, and colony counting.

Safety in the lab Services

TIP: Film made by the Ministry of Housing, Spatial Planning and the Environment (VROM) regarding safe microbiological technique and the GGO regulations.
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