About Laboratory Support & Media preparation
Laboratory Support & Media preparation (LEM) is responsible for the general supply of (specialized) lab media as well as for the collection and autoclaving of GGO waste and cleaning/sterilization of glassware. Read more about our services and feel free to get in touch with us for general or specific enquires, comments and suggestions.
Standard media available Services
The LEM makes a range of media that can be found in the cabinets alongside the laboratories on each floor. Please get in touch with a LEM staff member for special requests.
NB: All media is made using Demi water with the exception of PBS 10X, TAE 50X and TBE 10X that are made using MilliQ water
Tris-Glycine (1000 ml Schott bottles) Demi water |
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Litre |
Tris |
Glycine |
1 |
30 gr |
144 gr |
TBE 10x (1000 ml Schott bottles) MilliQ water (Ph 8.3) |
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Litre |
Tris Base |
Boorzuur |
EDTA |
1 |
109 gr |
60 gr |
4,65 gr |
Tris-HCL 0,5 M pH 6,8 (100 ml Schott bottles) MilliQ water |
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Litre |
TRIS |
1 |
60,55 gr |
Tris-HCL1,5 M pH 8,8 (250 ml Schott bottles) MilliQ water |
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Litre |
Tris |
1 |
181,65 gr |
TAE (50x, 1000 ml Schott bottles) MilliQ water |
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Litre |
Tris Base |
ACETIC ACID |
500 mM EDTA |
1 |
242 gr |
57.1 ml |
100 ml |
10x PBS (1000 ml Schott bottles, pH 6.5 - 6.8) MilliQ water * |
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Litre |
NaCl |
KCl |
Na2HPO4*H2O |
KH2PO4 |
1 |
80 gr |
2 gr |
13,8 gr |
2,6 gr |
1x PBS (1000 ml Schott bottles, pH 7.2 - 7.4) MilliQ water * |
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Litre |
NaCl |
KCI |
Na2HPO4*H2O |
KH2PO4 |
1 |
8 gr |
0,2 gr |
1,38 gr |
0,26 gr |
10% SDS stock (100 ml Schott bottles) MilliQ water |
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Litre |
SDS |
1 |
100 gr. UltraPure (TM) sodium Dodecyl Sulfate (SDS)
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L-B Agar (500 ml conical flasks) Demi water * |
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Litre |
PEPTON |
YEAST |
NaCl |
Agar |
1 |
10 gr |
5 gr |
8 gr |
15 gr |
L-B Medium (100 ml & 250 ml Schott bottles) Demi water * |
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Litre |
PEPTON |
YEAST |
NaCl |
1 |
10 gr |
5 gr |
8 gr |
2x TY medium (250 ml Schott bottles) Demi water * |
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Litre |
PEPTON |
YEAST |
NaCl |
1 |
16 gr |
10 gr |
5 gr |
* Autoclaved
Cryo storage facility Services
Liquid Nitrogen Level Control System (CRAS)
The M505CE is a Microprocessor based Liquid Nitrogen Level Control System with the following features and functions:- Level monitoring and automatic filling of refrigerators.
- Level display (High/Normal/Low).
- Temperature monitoring and display.
- Temperature Control
- Generation of local and remote alarms for:
- High Temperature
- High or Low liquid levels
- Sensor faults
- Failure of liquid nitrogen supply
- Lid left open
Contact persons Liquid Nitrogen Facility in case of ALARM
1. Ronald Scheffer |
+31 (0)24 361 42 57 |
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2. Martijn Verdoes |
+31 (0)24 361 05 57 |
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3. Jona Walk |
+31 (0)24 361 36 63 |
Contact persons Liquid Nitrogen Facility in case of management issues
1. Ben Joosten |
+31 (0)24 361 46 73 |
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2. Nicolai Giling |
+31 (0)24 369 36 52 |
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3. Bert Gerrits en bedrijf techniek |
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+31(0)24 361 33 83 / |
Freezer storage facility Services
As well as housing the technical facilities for the RIMLS research building, the 4th floor accommodates serveral facilities.- A back-up/emergency -80°C freezer is also available.
This freezer may be reserved (max 1 day). For more info contact medewerkersLEM.fysiol@radboudumc.nl tel. 14257.
Las 4.000 Odyssey Services
ImageQuant LAS 4000
ImageQuant LAS 4000 is a digital imaging system for sensitive, quantitative imaging of gels and blots, by chemiluminescence and fluorescence. A flexible combination of light sources and filters can be incorporated into the imager according to your needs.ImageQuant LAS 4000 is designed to quantitate the highly sensitive detection of proteins using Amersham ECL Western blotting reagents. A free combination of light sources and filters can be incorporated into the imager according to your needs.
The system consists of an intelligent dark box, camera, lens, and automated four-position filter wheel with a standard filter for EtBr and open positions for the filters delivered with the optional fluorescent light sources. Focusing, filters, illuminators, and exposure time are remotely controlled by a computer. Simple operation allows adjustment without having to open the cabinet. The intelligent light box provides automation and user safety. UV light sources are housed inside the cabinet, and can only be switched on when the door is closed.
A bright, wide aperture FUJINON™ F0.85 lens, specially developed for chemiluminescence imaging, projects sharp images onto a patterned CCD chip. By diagonally aligning octogonal pixels, the gap between pixels in the horizontal and vertical planes is reduced. This pattern allows an effective image resolution of 6.3 Mpixels.
The user-friendly control software has several capture modes for achieving optimal sensitivity and dynamic range including increment, repetition, and program modes. For image analysis, advanced and flexible ImageQuant TL software is available separately and comprises a dedicated suite of analysis applications to provide high levels of automation and accuracy in analysis of gels, blots, and colony counting.