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Mass spectrometers for glycomics
Our nanoLC-chip-QTOF can be used with different chip lay-outs, depending on the application. read moreMass spectrometers for glycomics
Our nanoLC-chip-QTOF can be used with different chip lay-outs, depending on the application. For peptide and glycopeptide profiling reversed phase C18 chips are available, for whole protein analysis C8 chips and for glycan profiling Carbograph chips. Several protocols for glycomics and glycoproteomics applications are available.
What is glycomics?
text to followGlycomic applications
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The high mass resolution of the QTOF allows annotation of protein glycoforms by C8-chip-QTOF.
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Whole protein glycoprofiling
The high mass resolution of the QTOF allows annotation of protein glycoforms by C8-chip-QTOF. We have recently established a method for analysis of the glycoforms of immunopurified serum transferrin (~80kDa), showing a variety of different glycan structures. -
N-glycans enzymatically released from glycoprotein preparations and biological fluids (e.g. serum, liquor) can be separated and analyzed using the Carbographchip.
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N-glycan profiling
N-glycans enzymatically released from glycoprotein preparations and biological fluids (e.g. serum, liquor) can be separated and analyzed using the Carbographchip. For interpretation of the data, in-house developed software is utilized in addition to the commercial data analysis software. A recent new innovative development is the use of a PNGase enzyme reactor for on-chip release of N-glycans and subsequent mass spec analysis. Both methods for N-glycan profiling can be applied for pure (recombinant) proteins and protein mixtures. -
Analysis of selected glycopeptides is possible on the C18 chip after off-line enrichment of glycopeptides.
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These methods are part of our current and future developments.
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Analysis of free glycans (oligosaccharides) is facilitated by labeling with a fluorescent tag for HPLC or mass spectrometry or with an isotopic derivative for quantitative measurements.
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Glycan (fluorescence) labeling
Analysis of free glycans (oligosaccharides) is facilitated by labeling with a fluorescent tag for HPLC or mass spectrometry or with an isotopic derivative for quantitative measurements. Both methods are available and are being applied to the analysis of free oligosaccharides in urine.
Other fields
Metabolomics
We have experience in metabolite measurements and hosts state-of-the-art equipment for targeted and untargeted metabolite analyses. read moreProteomics
We focus on mass spectrometry-based protein analyses and have proteomics expertise in a wide range of biological and analytical applications. read more
Radboudumc Technology Center Mass spectrometry
The Technology Center for Mass Spectrometry offers high-level expertise in bioanalytical mass spectrometry combined with in-depth knowledge on biomedical and clinical applications, embedded in a cutting-edge clinical and fundamental research environment.
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