Technology center Flow cytometry


Flow cytometry Expertise and services

At the Radboudumc extensive experience in flow cytometry is used in basic and translational research as well as clinical practice and clinical trials. We offer scientists advice and access to flow cytometry analyzers and sorters.

expertise and services

Flow cytometry Expertise and services

Flow cytometry is a sophisticated technique of single cell analysis. It simultaneously measures and analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. Some of the characteristics measured include a particle's relative size, granularity or internal complexity and relative fluorescence intensity.

Area of expertise

  • Flow cytometry used in basic and translational research as well as clinical practice and clinical trials.

Services

  • Access to flow cytometry analyzers and a flow cytometry sorter
  • Advice on multi-color experimental design and data interpretation
  • Personal assistance for analysis available by appointment
  • Extensive training on analyzers
  • Training and education in flow cytometry
  • High-end, supervised cell sorting

Fields of research

  • Immunology/Hematology
  • Genetic and metabolic diseases
  • Cell biology
  • Molecular biology
  • Microbiology
  • Infectious diseases
  • Gynaecology (sperm)
  • Biochemistry
  • Chemistry (small particles)

How can we help enhance your research?

Harry Dolstra PhD
+31 (0)24 361 97 53
Technology center representative

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Facility Flow cytometry

Radboudumc offers access to flow cytometry analyzers and a flow cytometry sorter.

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Applications

  • Immunophenotyping
  • Cell sorting based on fluorescent proteins
  • 4 way/populations sorting
  • Multiwell single cell sort 6-384 well plates
  • Nucleic acid analysis/ Cell cycle analysis
  • FRET measurements
  • Small particles analysis and sorting
  • Cell function analysis (calcium efflux)
  • Cytokines

Using our equipment

Users who have operated flow cytometry equipment will, after evidence of proficiency, be allowed to use the equipment unassisted. Individual and group training in the use of our equipment is available to allow for unassisted use in the future.

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Using our equipment

Where

NIG Building M379 room number 4.148 (route number 475).

When

Mondays through Fridays from 8:00 to 18:00 hrs.

Reservations

You can make a reservation request using your own Outlook agenda and invite the cell sorter.
  • In outlook select calendar (agenda) and then new (nieuw)
  • Select guide card invite attendees (deelnemers uitnodigen)  and send to (aan) Vz apparatuur Laboratoriumgeneeskunde LH flowcytometer Aria (VZ00329) and add this to required (vereist).
  • You can make this appointment. Select scheduling (planning). The bars indicate when the cell sorter is busy. Select the time you need to sort you samples.
  • Select appointment (afspraak). Mention your name and phone number under subject (onderwerp).
  • Finally, press send (verzenden).

Training

Contact Head Operator Rob Woestenenk at +31 (0)24 361 52 32 for instructions.

What is flow cytometry?

Flow cytometry is a sophisticated technique of single cell analysis.

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What is flow cytometry?

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Flow cytometry is a sophisticated technique of single cell analysis. It simultaneously measures and analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. Some of the characteristics measured include a particle's relative size, granularity or internal complexity and relative fluorescence intensity.


  • A flow cytometer is composed of three main systems: fluidics, optics and electronics. The fluidics transports the particles in a stream to the laser beam for interrogation. The optics consists of lasers to illuminate the particles in the sample stream and optical filters to direct the resulting light signals to the appropriate detectors. The electronics convert the detected light signals into digital signals that can be processed by the computer.

    Monoclonal antibodies tagged with a fluorescent dye can be used to identify a particular cell type based on individual surface, cytoplasmic, or nuclear antigens. Different mixtures of fluorochromes can be used to distinguish sub-populations. Dyes, which bind directly to cell components such as DNA and RNA, are used as well.

    The flow technology provides a powerful and sensitive tool for investigating the structure and function of cells, bacteria and other particles. These measurements can be made at flow rates of 100-20.000 cells per second.

Our experts


Research groups Flow cytometry

Discover some of our research that is related to flow cytometry.

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