Radboudumc Technology Center Genomics

News and Events

Event: 09 September 2025
On 9 September at 7:30 AM PT / 4:30 PM CET, the webinar in the Bionano In Motion Series will explore how OGM is reshaping cytogenetic research. Dr. Kornelia Neveling from Radboudumc will present her team’s research on OGM in a talk entitled “Optical Genome Mapping at Radboudumc: Leukemia, Rare Diseases and Repeat Expansions''. See LinkedIn for an impression of this event. Sign up here. 

Event: 27 March 2024
With the arrival of the 2nd Revio, we are offering our long-read sequencing services to external parties. On Tuesday, March 19, we organized an event in collaboration with PacBio, “Radboud PacBio Day". See LinkedIn for an impression of this event.

News: 17 April 2023
Reportage NRC handelsblad: 'Is het een schimmel, is het een virus? Sequencers profileren ziektekiemen razendsnel' hierin vertelt o.a. bio-informaticus Jordy Coolen over het sequencen op de apparaten van ons technology center.

News: 22 December 2022
PacBio long-read sequencing in collaboration with the Senckenberg Research Institute and Natural History Museum

Genome assembly is a valuable resource for future conservation of species that are threatened by extinction. NGS technologies have become globally established for the study of systematic-phylogenetic issues. We collaborate with the Senckenberg Research Institute and Natural History Museum and facilitate PacBio long-read sequencing. If interested read these recent publications:

• Chueca et al. De novo Genome Assembly of the Raccoon Dog (Nyctereutes procyonoides). Front Genet. 2021 Apr 29;12:658256. PMID: 33995489.
• Blumer et al. Gene losses in the common vampire bat illuminate molecular adaptations to blood feeding. Sci Adv. 2022 Mar 25;8(12):eabm6494. PMID: 35333583.
• Merges et al. Virus diversity in metagenomes of a lichen symbiosis (Umbilicaria phaea): complete viral genomes, putative hosts and elevational distributions. Environ Microbiol. 2021 Nov;23(11):6637-6650. PMID: 34697892.
• Winter et al. A Chromosome-Scale Genome Assembly of the Okapi (Okapia johnstoni). J Hered. 2022 Oct 21;113(5):568-576. PMID: 35788365.

News: 07 November 2022
PacBio long read amplicon sequencing

In our recent study by Daan Viering et al, PacBio long read amplicon sequencing identified 46 previously undetected exonic and intronic variants in 95 pre-screened patients with suspected Gitelman syndrome. This paper highlights that intronic pathogenic variants explain an important part of the missing heritability in Gitelman syndrome, and shows the advantage of long read amplicon sequencing for the detection of these so far hidden variants.

News: 13 October 2022
Minister Ernst Kuipers brengt werkbezoek aan het Genomics Technology Center

Op woensdag 12 oktober heeft minister Ernst Kuipers van het Ministerie van Volksgezondheid, Welzijn en Sport een bezoek gebracht aan ons Technology Center.

Instruments and equipment

In our lab we have a variety of instruments and equipment available.

•    1x - Novaseq X Plus (Illumina)
•    3x - NovaSeq6000 (Illumina)
•    2x - NextSeq500 (Illumina)
•    2x - MiniSeq (Illumina)
•    2x - Revio (Pacific Biosciences)
•    2x - Sequel IIe (Pacific Biosciences)
•    1x - Stratys (Bionano)
•    2x - Saphyr (Bionano)
•    1x - GridION (Oxford Nanopore)
•    2x - ABI 3730XL analyzer (ThermoFisher)

Services

For up to date information about sequencing projects, practical questions or prices, please contact us via GTC@radboudumc.nl

Short Read – WGS 

Long Read - WGS

With the Sequel IIe system (Pacific Biosciences) we are able to generate long sequencing reads for WGS applications. 

Hi-Fi Mode:

For generating sequencing reads with an average length of 15-20kb with a minimum accuracy of 99.9%
     - SNV, InDel and SV Detection
     - De Novo assembly

We can offer the following services:
•    Preparing whole genome libraries from gDNA and sequencing
•    Sequencing only of finished SMRTbell libraries