21 January 2019

Martijn Huijnen, theme Mitochondrial diseases, and colleagues developed COmplexome Profiling ALignment (COPAL) to systematically asses the effect of Barth syndome on mitochondrial protein complexes.

They published their findings in Bioinformatics.

Complexome profiling combines native gel electrophoresis with mass spectrometry to obtain the inventory, composition and abundance of multiprotein assemblies in an organelle. Applying complexome profiling to determine the effect of a mutation on protein complexes requires separating technical and biological variations from the variations caused by that mutation.

They have developed the COmplexome Profiling ALignment (COPAL) tool that aligns multiple complexome profiles with each other. It includes the abundance profiles of all proteins on two gels, using a multidimensional implementation of the dynamic time warping algorithm to align the gels. Subsequent progressive alignment allows them to align multiple profiles with each other. They tested COPAL on complexome profiles from control mitochondria and from Barth syndrome (BTHS) mitochondria, which have a mutation in tafazzin gene that is involved in remodelling the inner mitochondrial membrane phospholipid cardiolipin. By comparing the variation between BTHS mitochondria and controls with the variation among either, they assessed the effects of BTHS on the abundance profiles of individual proteins. Combining those profiles with gene set enrichment analysis allows detecting significantly affected protein complexes. Most of the significantly affected protein complexes are located in the inner mitochondrial membrane (MICOS, prohibitins), or are attached to it (the large ribosomal subunit).

Related news items


Stuttering DNA orchestrates the start of the mosquito’s life

9 April 2020

A certain type of junk DNA that is found in mosquitoes and which repeats itself dozens of times, known as ‘satellite DNA’, has now been shown to play an essential role in the early development of mosquito embryos. Ronald van Rij and colleagues published their findings in Nature.  

read more

Webinar for researchers 20 April 17:00 hrs

9 April 2020

The corona crisis has an impact on our research every day. Therefore we organize a webinar specifically aimed at researchers. Do you have specific topics or questions that you would like to see reflected in the webinar? Let us know via research@radboudumc.nl.

read more

The ICI Amsterdam-Leiden-Nijmegen cancer vaccine consortium

7 April 2020

The Chemical Immunology group from Martijn Verdoes, theme Nanomedicine, joined forces with two Organic Chemistry groups and a Chemical Biology group from Leiden University, and Immunology groups at the AmsterdamUMC and the LUMC to form a consortium to develop new cancer vaccines.

read more

Martinus van Marum prize for Tom Schirris

3 April 2020

This award of € 12,500 is intended as an incentive prize for researchers who have obtained their PhD degree in 2015 or later. The jury, consisting of members of the Society, came to this decision unanimously.

read more

Crowdfunding for research and innovations

3 April 2020

Radboud university medical center and Radboud University collaborate on initiatives in the fight against corona.

read more

New scientific integrity contact Bart Kiemeney

2 April 2020

Bart Kiemeney will replace Gerhard Zielhuis as one of the contact persons scientific integrity.

read more