21 January 2019

Martijn Huijnen, theme Mitochondrial diseases, and colleagues developed COmplexome Profiling ALignment (COPAL) to systematically asses the effect of Barth syndome on mitochondrial protein complexes.

They published their findings in Bioinformatics.

Complexome profiling combines native gel electrophoresis with mass spectrometry to obtain the inventory, composition and abundance of multiprotein assemblies in an organelle. Applying complexome profiling to determine the effect of a mutation on protein complexes requires separating technical and biological variations from the variations caused by that mutation.

They have developed the COmplexome Profiling ALignment (COPAL) tool that aligns multiple complexome profiles with each other. It includes the abundance profiles of all proteins on two gels, using a multidimensional implementation of the dynamic time warping algorithm to align the gels. Subsequent progressive alignment allows them to align multiple profiles with each other. They tested COPAL on complexome profiles from control mitochondria and from Barth syndrome (BTHS) mitochondria, which have a mutation in tafazzin gene that is involved in remodelling the inner mitochondrial membrane phospholipid cardiolipin. By comparing the variation between BTHS mitochondria and controls with the variation among either, they assessed the effects of BTHS on the abundance profiles of individual proteins. Combining those profiles with gene set enrichment analysis allows detecting significantly affected protein complexes. Most of the significantly affected protein complexes are located in the inner mitochondrial membrane (MICOS, prohibitins), or are attached to it (the large ribosomal subunit).

Related news items


Frank Walboomers 25-years work anniversary at Radboudumc

17 September 2020

Frank Walboomers, associate professor at the research group Regenerative Biomaterials at the Dept. of Dentistry (theme Reconstructive & Regenerative Medicine), celebrated his 25th work anniversary at Radboudumc.

read more

Transfer of new anti-hepatitis C drugs across the human placenta

9 September 2020

In a recent publication in American Journal of Obstetrics and Gynecology, researchers from the Departments of Pharmacology and Toxicology, Pharmacy, Obstetrics and Gynecology, Gastroenterology and Hepatology, investigated the placental passage of two such drugs for the first time.

read more

Does the COVID-19 cytokine storm exist? Research may have an impact on the chances of success of a specific treatment

4 September 2020

Following the measurement of several important cytokines in patients with COVID-19 and various other severe diseases, researchers at Radboud university medical center now show that COVID-19 is not characterized by a cytokine storm.

read more

The European Joint Program on Rare Diseases organizes a General Assembly meeting

2 September 2020

The European Joint Program on Rare Diseases (EJP RD) organizes its second General Assembly (GA) and Consortium Meeting from 14 to 18 September 2020 (online).

read more

Effects of pathogenic fungus screened internationally new fungus Candida auris first observed in 2009

1 September 2020

Mariolina Bruno, Simone Kersten and Mihai Netea, and colleagues have discovered that the human immune system recognizes the fungus well. Their study has been able to pin-point the fungus' Achilles heel for new, effective drugs. This has recently been published in Nature Microbiology.

read more

Tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culture

26 August 2020

Carl Figdor and colleagues showed in Nature Communications that the tumour microenvironment (TME) forms a major obstacle in effective cancer treatment and for clinical success of immunotherapy.

read more