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DE
14 March 2019
Outline of this thesis
This thesis aims to further disclose the causal relationship between hypomagnesemia and T2D.
In Chapter 2 the prevalence of hypomagnesemia and the extent of urinary Mg2+ wasting was studied in a cohort of patients with advanced T2D. Clinical and laboratory factors were associated to changes in plasma Mg2+ levels.
Chapter 3 aimed to study the metabolic consequences induced by hypomagnesemia in a T2D mouse model, using a Mg2+ deficient high fat diet.
In Chapter 4 the same dietary intervention was used to study the effects of a high fat diet on Mg2+ homeostasis. This chapter also studied the consequences of hypomagnesemia on the kidney.
In Chapter 5 we investigated the effect of metformin treatment on Mg2+ homeostasis in genetically modified mice (db/db mice), which suffer from T2D. The rationale for this study was based on the association between plasma Mg2+ and the use of metformin in T2D patients of the PARELSNOER cohort.
In Chapter 6 serum Mg2+ levels were correlated to alterations in the lipid profile of overweight individuals. This chapter further studied the binding of Mg2+ to negatively charged FFA molecules to unravel the inverse relationship between triglycerides and Mg2+. Acute increases in blood lipid levels were achieved using oral loading of olive oil and cream in mice and human subjects, respectively. The main results of this thesis are summarized in Chapter 7.
In Chapter 8 the main findings are put into perspective. In this chapter we also discuss the clinical translation of the results and formulate recommendations for clinical practice. Finally, potential directions for further research are provided.
Click here to access Steef' thesis digitally.
Steef Kurstjens obtained his Ph.D. degree in the Medical Sciences of the Radboud University Nijmegen after the successful defense of his thesis.
Outline of this thesis
This thesis aims to further disclose the causal relationship between hypomagnesemia and T2D.
In Chapter 2 the prevalence of hypomagnesemia and the extent of urinary Mg2+ wasting was studied in a cohort of patients with advanced T2D. Clinical and laboratory factors were associated to changes in plasma Mg2+ levels.
Chapter 3 aimed to study the metabolic consequences induced by hypomagnesemia in a T2D mouse model, using a Mg2+ deficient high fat diet.
In Chapter 4 the same dietary intervention was used to study the effects of a high fat diet on Mg2+ homeostasis. This chapter also studied the consequences of hypomagnesemia on the kidney.
In Chapter 5 we investigated the effect of metformin treatment on Mg2+ homeostasis in genetically modified mice (db/db mice), which suffer from T2D. The rationale for this study was based on the association between plasma Mg2+ and the use of metformin in T2D patients of the PARELSNOER cohort.
In Chapter 6 serum Mg2+ levels were correlated to alterations in the lipid profile of overweight individuals. This chapter further studied the binding of Mg2+ to negatively charged FFA molecules to unravel the inverse relationship between triglycerides and Mg2+. Acute increases in blood lipid levels were achieved using oral loading of olive oil and cream in mice and human subjects, respectively. The main results of this thesis are summarized in Chapter 7.
In Chapter 8 the main findings are put into perspective. In this chapter we also discuss the clinical translation of the results and formulate recommendations for clinical practice. Finally, potential directions for further research are provided.
Click here to access Steef' thesis digitally.
