Research Facilities Optical imaging

About our equipment

Optical imaging offers unique possibilities for in vitro and in vivo imaging applications, especially in the context of molecular imaging.

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About our equipment

Optical imaging offers unique possibilities for in vitro and in vivo imaging applications, especially in the context of molecular imaging. With the IVIS Lumina imaging system, present in PRIME, it is possible to identify disease pathways, determine mechanisms of action, evaluate drug compounds, and monitor their effects on disease progression in living animals non-invasively.

This imaging system is highly sensitive and can image fluorescent  and/or bioluminescent reporters and probes both in vivo and in vitro. This imaging module is user friendly and allows rapid (1-10 minutes) quantitative assessment of fluoresceny in 3-5 mice or 2 rats simultaneously. The advantage is that a huge amount of biological tools (bioware) are commercially available such as trangenic reporter animals, reporter tumor cells, microorganisms , gene reporters, enzymatic cleavable substrates and quantum dots. In-house near infra red flurescent probes are produced for fluorescence imaging. 

Applications in PRIME

The IVIS Lumina in PRIME can be used for several applications.

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Applications in PRIME

The IVIS Lumina in PRIME can be used for the following applications:

  • to study the physio, pathological and molecular processes in real time and longitudinally;
  • for diagnostics, pharmacokinetics, pharmacodynamics, and drug screening in preclinical studies.

This imaging module is used in PRIME for:

  • Tumor imaging with fluorescence imaging
  • Skin infection models
  • Wound healing
  • Malaria infection / immunization 
  • Disease-inducible promoters for inflammation 
  • Rheumatoid arthritis and osteoarthritis models 


IVIS Lumina Caliber

Standard fluorescent filter sets

Filter set

Label

Background Passband

(nm)

Excitation Passband (nm)

Emission Passband (nm)

Dyes, Fluorescent proteins, and Quantum dots

1

Green

410-440

445-490

515-575

GFP, EGFP, FITC

2

Red

460-490

500-550

575-650

DsRed, PKH26, Qdot® 605

3

Far-red

580-610

615-665

695-770

Cy5.5, Alexa Fluor, Qdot®705

4

NIR

665-695

705-780

810-885

ICG, Qdot® 800


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