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In this blog-interview series, we present the Radboudumc Technology Centers (RTCs) – your gateway to technological expertise, state-of-the-art equipment, and invaluable resources. In each part we spotlight one of the 19 RTCs housed within our organization.
Today, we have the pleasure of sitting down with Silvia Albert on behalf of the Stem Cell Technology Center (SCTC), based within the Department of Human Genetics.
Silvia, could you tell us about who you are?
“Certainly! We are a small enthusiastic team consisting of experts—Anke, Tessa, Louet, and Julia—driving the SCTC's operations. Our focus lies in leveraging stem cells for cutting-edge research in disease modeling.”
Can you walk us through the services you provide?
“In order to study the role of genetic mutations, in vitro models are needed to mimic what happens in the human body. To get the cells for the disease models, we must first produce stem cells or induced Pluripotent Stem (iPS) cells from several types of adult cells (e.g. from blood or urine) via a process called reprogramming. The next step is to turn the iPS cells into several cell types through a differentiation process. Reprogramming and differentiation are our main services, but we also offer many other services.”
Can you give us examples of the differentiation process you mentioned?
“Yes, sure. For instance, we take stem cells reprogrammed from patient's urine or blood and guide them into becoming beating heart cells (cardiomyocytes), brain cells (neurons), or various types of eye/retinal cells. These transformed cells serve as the building blocks for our in vitro models.”
That's intriguing. And can you tell us more about how you can help with genetic manipulations?
“We utilize Crispr/Cas technology for genetic manipulation, correcting or introducing specific mutations. We also handle cell culture scaling, applying compounds for drug testing, and thorough characterization to validate gene expression and protein production. All our cell lines come with a certificate of analysis, a document where all the analyses that we performed on the cells are reported.
Being part of the Human Genetics Department stimulates us to constantly evolve, in order to offer state-of-the-art techniques. In collaboration with the division of diagnostics, we are able check the cell´s DNA to make sure that no abnormalities were introduced during the cell culture.”
How does SCTC lend a helping hand to researchers in their work?
"We're here as a collaborative partner for stem cell culture and troubleshooting. Our services cover:
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Adult cell reprogramming into induced pluripotent stem cells (iPSCs)
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iPSC differentiation into several cell types or organoids
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iPSC genetic manipulation, using the Crispr/Cas technology
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iPSC characterization, to prove the undifferentiated and the pluripotency state
On top of these services, we also assist in grant applications, scientific writing, and offer personalized workshops. Plus, we've organized a summer school at Radboud University, guiding students in reprogrammed stem cell culture."
For researchers interested in SCTC's services, what's the next step?
"If you're keen to utilize our services, you can just email us with your inquiries. We will then answer your questions and discuss with you your preferences and explain to you how the process works. Creating iPS cell lines is time consuming and there are only limited spots available per month for reprogramming, so make sure to start your request on time."
So, one email will do. That's great! And where can researchers find more information about your RTC?
"Even for inquiries and updates, you can email us or else you can visit our website. We also share our latest developments on Instagram, keeping our community informed and engaged. Although we are bit spread around, you can always come and visit us at route 855. We'll be around!"
Nice to hear about your commitments to stay engaged with your community. Thank you very much, Silvia, for sharing your expertise in advancing stem cell research at Radboudumc.
"Thank you! It's been a pleasure."
Picture: Main Entrance
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Interview by Hasan Erkan
Editor Radboudumc Research Newsletter
You can find all editions of our blog series on this page
