News items Epigenetics Immunization is passed on to offspring
19 October 2021

Does an infection affect the immunization of subsequent generations? It does, according to research now published in Nature Immunology. Mouse fathers who had overcome an infection with fungi passed on their improved protection to their offspring across several generations. A broader improved immune response was being passed on to the descendants as well.

Scientific studies show that environmental influences can pass on to the next generation by means of epigenetic changes. Epigenetic changes modify the activation of certain genes, but not the genetic code itself. It has been shown in plants and invertebrates that epigenetics changes – such as resistance to infections – can be passed on to next generations. A research team from the Radboud University Medical Center Nijmegen (Netherlands), the University of Bonn, Saarland University (both Germany), the University of Lausanne (Switzerland) as well as the National and Kapodistrian University of Athens (Greece) has now investigated whether effects on the innate immune system (trained immunity) in mammals are also passed on to the next generations.

Infection with fungi trains the immune system of mice

The researchers infected male mice with Candida albicans (fungi). After recovering from the infection, the animals were mated with completely healthy females. The researchers compared the resulting offspring with offspring from pairs of mice that were not infected with Candida. In order to investigate the status of the immune system experimentally, the team infected the males of the subsequent generation of mice with coliform bacteria. "The offspring of the male mice previously exposed to Candida were significantly better protected from a subsequent E. coli infection than the progeny of the uninfected male mice," reports Prof. Dr. Mihai G. Netea of the Radboud Center for Infectious Diseases. This effect was still evident in the next generation.

Epigenetically rewired

How does this transmission of immunization to subsequent generations work? The team examined typical immune cells such as monocytes or neutrophils. No differences were detectable between the offspring of Candida-infected male mice and the non-infected control group. However, in the offspring of the previously infected mouse fathers, the MHC class II complex was upregulated, which activates parts of the immune system. In addition, the activity of genes involved in inflammation was also found to be upregulated in the offspring of Candida-infected male mice. In the offspring of fathers previously infected with C. albicans, it was found that genes associated with inflammation were easier to read in monocyte progenitors than in sons of uninfected fathers. "This shows that monocyte precursors are epigenetically rewired if the fathers have previously undergone infection with C. albicans," Prof. Dr. Andreas Schlitzer of the LIMES Institute at the University of Bonn summarizes.

Shift in gene activity is detectable in sperm

How does the transmission of this information to the next generation take place? In cooperation with Saarland University, the researchers investigated the gene activity of the sperm of mouse fathers infected with Candida. They analyzed the extent to which methyl groups blocked access to genes in order to de-activate them. "A shift in gene markers was evident here," says Prof. Dr. Jörn Walter of Saarland University. Offspring of Candida-infected male mice showed fewer gene blockages in gene regions important for inflammatory processes and monocyte maturation. How the information about the sperm markings reaches the bone marrow, the birthplace of many immune cells, still needs to be explored in further studies.

In mice and men?

"The study is the first to show in mammals that adaptations to infectious diseases are also passed on to the offspring," Netea says. In contrast to the classical theory of evolution, which assumes slow adaptation through changes in the genetic code, this involves very rapid changes via the epigenetic regulation of gene activities, irrespective of the genetic code. The researchers do not yet know whether the findings obtained in mice can also be transferred to humans. "But we are assuming that this is the case," Schlitzer says. "The immune system mechanisms and cells involved are very similar in mice and humans."


Publication in Nature Immunology: Transmission of trained immunity and heterologous resistance to infections across generations - Natalie Katzmarski, Jorge Domínguez-Andrés, Branko Cirovic, Georgios Renieris, Eleonora Ciarlo, Didier Le Roy, Konstantin Lepikhov, Kathrin Kattler, Gilles Gasparoni, Kristian Händler, Heidi Theis, Marc Beyer, Jos W. M. van der Meer, Leo A. B. Joosten, Jörn Walter, Joachim L. Schultze, Thierry Roger, Evangelos J. Giamarellos-Bourboulis, Andreas Schlitzer, Mihai G. Netea: DOI: 10.1038/s41590-021-01052-7

More information

Pieter Lomans


neem contact op

Related news items

Yvonne Schoon appointed professor by special appointment 'The right care for the right older person' 

6 December 2021

Yvonne Schoon has been appointed professor by special appointment 'The right care for the right older person' at the Radboudumc / Radboud University as of 1 October 2021.

read more

David Burger top scientist on anti-HIV medication

6 December 2021

Professor of Clinical Pharmacy David Burger, affiliated with the Radboudumc Pharmacy, is in the list of top scientists in the field of anti-HIV medication.

read more

How much should we exercise to live healthier lives? Research into the relationship between exercise, heart disease and mortality

3 December 2021

It has long been known that exercise reduces the risk of many chronic diseases. However, we do not yet know exactly how much exercise is necessary to achieve health benefit.

read more

KNAW Early Career Award for Martine Hoogman

2 December 2021

Martine Hoogman has been awarded a KNAW Early Career Award. The prize, a sum of 15,000 euros and a work of art, is aimed at researchers in the Netherlands who are at the start of their careers and have innovative, original research ideas.

read more

T-Guard can reset immune system

1 December 2021

For 20 years, the Nijmegen based Radboudumc spin-off company Xenikos has been working on a drug that can reset the immune system. This reset will save lives of seriously ill patients. Now, after years of hard work, the moment of truth has arrived for T-guard.

read more

Thomas van den Heuvel wins Stairway to Impact Award for safer pregnancies using AI Award for safer pregnancies using AI

1 December 2021

Radboudumc researcher Thomas van den Heuvel receives the Stairway to Impact Award from Dutch Research Council NWO. He receives this prize for the development of the BabyChecker, a smartphone application that allows midwives to make ultrasounds during pregnancies.

read more