22 May 2017

Immune cells secrete a wide array of cytokines and express surface receptors to communicate with their environment.

In cells, these molecules are transported by vesicles that bud off from the one intracellular compartment and move to, dock, and finally fuse with another compartment. SNARE proteins are essential for membrane fusion for all these steps of vesicle trafficking. However, the main regulators of intracellular trafficking in dendritic cells are widely understudied, because SNAREs are functionally redundant and highly abundant within cells.

Danielle Verboogen, group Geert van den Bogaart, theme Cancer development and immune defense, developed a Förster resonance energy transfer (FRET)-based fluorescence lifetime imaging microscopy (FLIM) technique to measure SNARE-complex formation in primary human blood cells. Their data shows that secretion of interleukin-6 by dendritic cells is regulated not only biosynthetically, but also at the level of intracellular trafficking via complex formation of specific sets of SNARE proteins.

Verboogen, D.R.J., González Mancha, N., Ter Beest, M., & van den Bogaart, G. (2017) Fluorescence lifetime imaging microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation. Elife. 6: e23525.

Related news items

ZonMw Open Competition Grant for Annemiek van Spriel and Piet Gros

15 June 2021 Annemiek van Spriel, theme cancer development and immune defense, together with Piet Gros, Dept. of Chemistry, Utrecht University obtained a ZonMw Open Competition Grant to investigate IL-6 receptor structure and signalling in tumor cells. read more

HFSP Grant for Johannes Textor

30 March 2020 Johannes Textor, theme Cancer development and immune defense, has been awarded a program grant of 1 million US dollars by The Human Frontier Science Program (HFSP) to investigate how T cells navigate extremely dense environments using experiments, modeling and methods from pedestrian dynamics. read more

Human pDCs attract cytolytic lymphocytes in anti-cancer vaccination

6 February 2020 RIMLS researchers Jasper van Beek and Jolanda de Vries, theme Cancer development and immune defense, discovered that pDCs can efficiently recruit cytolytic immune effector cells, a property that could be exploited in anti-cancer vaccines. They have published these findings in Cell Reports. read more

ZonMw funding for teams of Michiel Vermeulen and Jolanda de Vries

4 February 2020 The awarded research projects of RIMLS researchers Michiel Vermeulen, Jolanda de Vries, Gerty Schreibelt and Martijn Verdoes and colleagues will initiate new research lines and collaborations between various research groups. read more

Niels Stensen Fellowship for Jorieke Weiden

6 January 2020 Jorieke Weiden was recently awarded a grant from the Niels Stensen Fellowship. Since the 1960s, the Niels Stensen Fellowship has been awarded annually to outstanding researchers who recently received their PhD to enable them to gain research experience at a top university or institute abroad. read more

Galectin-9 controls plasma membrane integrity of dendritic cells

16 December 2019 Laia Querol Cano, theme Cancer development and immune defense, has discovered how galectin-9, a sugar-binding membrane organiser, governs dendritic cell function by intracellularly modulating actin cytoskeleton. These findings are published in the latest issue of iScience. read more