Techniques Radiotherapy & Onco­Immunology Laboratory

The biology of tumors is studied at the macroscopic (PET) and microscopic (cell, subcellular) level. The aim is to compare different functional imaging modalities for the same tumor.
The focus is on:

  • Quantitative immunohistochemistry
  • Cell culture systems
  • Molecular Immunology

Quantitative immunohistochemistry

The backbone of this system are:

  • the vascular architecture (9F1 or CD31/24)
  • tumor blood perfusion (Hoechst 33342)
  • exogeneous markers/indicators of hypoxia (CA-IX, pimonidazole)

This system can be extended by:

  • proliferation (BrdUrd, IdUrd, Ki67)
  • growth factors ((p)EGFR, (p)AKT)
  • DNA damage (gamma-H2AX, 53BP1)

At the microscopic level, the tumor biology is studied in a quantitative manner with preservation of the tissue architecture and spatial associations. Therefore, we have developed a system for co-registration and quantitative analysis of micro-environmental phenotypic tumor characteristics. The method is based on immunehistochemical detection of multiple fluorescent signals in complete tissue sections.

Quantification

Components of our digital imaging systems: 

  • multi-color fluorescence microscopy
  • computer-controlled scanning and imaging system:
    • CCD camera
    • motorised scanning stage
    • image acquisition and analysis software

Cell culture systems

Our laboratory has permission for genetically modified organisms ('ML-II').
The facilities are:

  • Western Blotting
  • Isolation of RNA and DNA
  • Horizontal gel electrophoreses
  • Incubations at 0.1-20% oxygen (Hypoxystation)

Molecular Immunology

Information will be given shortly.


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