Department Radiation Oncology


About us

By developing predictive profiles based on micro-environmental tumor characteristics, we expect to better be able to guide treatment selection and optimization for patients on an individual basis.

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About us

Our experimental and clinical research program is aimed at:

Molecular Immunology

Dendritic cells (DC) are the professional antigen presenting cells (APC) of our immune system. They are able to initiate immune responses against pathogens or tumors, but also have the capacity to prevent (auto)-immune responses harmful to the host. My research is centered around the molecular and functional analysis of DC in mouse and man. Applying different molecular approaches at the genomic and proteomic level a set of novel DC-antigens have been identified, including chemokines (DC-CK1, CXCL16), a novel multiple membrane spanning receptor (DC-STAMP), a transcription regulator (DC-SCRIPT). Knowledge regarding DC-immuno-biology is essential for the development and design of DC-based vaccines in mouse models as well as in clinical studies in cancer patients. More recently, regulatory T cells that are crucially involved in balancing the immune system are studied at the molecular and functional level as well as in immunotherapy of cancer.

Vascular Architecture and Microenvironmental Parameters

An important objective is the development of predictive profiles based on Vascular Architecture and Microenvironmental Parameters (VAMP). The ultimate goal is to provide a mechanistic basis for the optimization of treatments that combine radiotherapy with novel biological modifiers and for the development of patient selection strategies.
 

Current topics we focus on

  • analyzing EGFR signaling related to radiation resistance (PI3-K/AKT pathway related to tumor vasculature)
  • proliferation and hypoxia involvement
  • non-invasive imaging of the tumor microenvironment (vasculature, hypoxia, proliferation and the EGFR)
  • assessment of endogenous markers related to tumor cell metabolism (lactate, monocarboxylate transporters etc)
Furthermore, clinical therapy could be much improved when the knowledge gathered in fundamental immunological studies is translated into clinical immunotherapy studies. In this light, our research interests focuses on the molecular analysis of professional antigen presenting dendritic cells and Myeloid and T-regulatory cells, and their function in the immune system in health and disease.

Contact

Henri Marres PhD
+31 (0)24 361 45 15
head of research

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Our researchers Radiation Oncology

A list of researchers connected to this department.

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Research groups Radiation Oncology

Discover some of our research that is related to radiation oncology.

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Themes



Techniques Radiotherapy & Onco­Immunology Laboratory

The biology of tumors is studied at the macroscopic (PET) and microscopic (cell, subcellular) level. The aim is to compare different functional imaging modalities for the same tumor.
The focus is on:
  • Quantitative immunohistochemistry
  • Cell culture systems
  • Molecular Immunology

Quantitative immunohistochemistry

The backbone of this system are:
  • the vascular architecture (9F1 or CD31/24)
  • tumor blood perfusion (Hoechst 33342)
  • exogeneous markers/indicators of hypoxia (CA-IX, pimonidazole)
This system can be extended by:
  • proliferation (BrdUrd, IdUrd, Ki67)
  • growth factors ((p)EGFR, (p)AKT)
  • DNA damage (gamma-H2AX, 53BP1)
At the microscopic level, the tumor biology is studied in a quantitative manner with preservation of the tissue architecture and spatial associations. Therefore, we have developed a system for co-registration and quantitative analysis of micro-environmental phenotypic tumor characteristics. The method is based on immunehistochemical detection of multiple fluorescent signals in complete tissue sections.

Quantification

Components of our digital imaging systems: 
  • multi-color fluorescence microscopy
  • computer-controlled scanning and imaging system:
    • CCD camera
    • motorised scanning stage
    • image acquisition and analysis software

Cell culture systems

Our laboratory has permission for genetically modified organisms ('ML-II').
The facilities are:
  • Western Blotting
  • Isolation of RNA and DNA
  • Horizontal gel electrophoreses
  • Incubations at 0.1-20% oxygen (Hypoxystation)

Molecular Immunology

Information will be given shortly.


Collaborations

Netherlands

  • Radboud university medical center, Dept. of Radiology: Prof. dr. A. Heerschap
    MR-imaging
  • Radboud university medical center, Dept. of Nuclear Medicine: Prof. dr. O.C. Boerman
    PET-scanning
  • University of Maastricht, Dept. of Experimental Radiation Oncology: Prof. dr. Ph. Lambin
    Hypoxic cytotoxins

Abroad

  • University of Aarhus: M. Busk
    Comparison of AZA PET and IHC
  • University of Oslo: D.R. Olsen & K. Roe
    Comparison of hypoxia/perfusion imaging with functional MRI
  • University of Oxford: Dr. E.J. Bernhard
    Mechanisms that contribute to radiation survival of tumour cells
  • University of Hamburg/Technische Universitat Dresden: Prof. dr. M. Baumann
    Molecular targeting of tumors during irradiation
  • University of Auckland (NZ): Prof. W. Wilson
    Enhancing tumour hypoxia for therapeutic gain
  • University of Chicago: Prof. G. Karczmar
    High spectral and spatial resolution MR imaging, co-registration with tissue sections, EPR hypoxia imaging
  • University of North Carolina, Dept. Radiation Oncology: Prof. J.A. Raleigh
    Hypoxic cell markers
  • Ontario Cancer Institut: Prof. dr. B. Wouters
    Molecular basis of signalling pathways that influence tumor response
  • UT Southwestern Medical Center (Dallas, Texas): P.M. Medin
    Tolerance of pig spinal cord for radiosurgery
  • University of Wisconsin: D.L. Wheeler & P.M. Harari
    EGFR & IGFR blockers

Radboud Institute for Health Sciences

This department is affiliated with RIHS. The research at this institute aims to improve clinical practice and public health.

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Radboud Institute for Molecular Life Sciences

This department is affiliated with RIMLS. Their main aim is to achieve a greater understanding of the molecular mechanisms of disease.

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Patient care

This department also offers patient care.

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Getting there

Entrance: Radboud Institute for Molecular Life Sciences
Route: 874

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Getting there

Visiting address

Radboud Institute for Molecular Life Sciences
Geert Grooteplein 28
6525 GA Nijmegen

Directions

Enter building at: Radboud Institute for Molecular Life Sciences
Follow route 874
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