Flow cytometry Expertise and services

Flow cytometry is a sophisticated technique that allows interrogation of few to millions of single cells or. Multiple physical characteristics of each individual cell/particle are simultaneously measured, and expression levels of antigens can be detected using fluorescently labelled antibodies. Measured characteristics include amongst others the relative size, granularity or internal complexity, and relative fluorescence intensity of the cell/particle. Our platform offers scientists advice on pre-analysis sample preparation, panel design, quality controls, instrument set-up, data acquisition and data analysis and visualization. Furthermore, we provide access to high-end flow cytometry analyzers and sorters.

Area of expertise

  • Flow cytometry used in basic and translational research as well as clinical practice and clinical trials.
  • Flow cytometry-based data acquisition of cells or small particles.
  • Sorting of cells or small particles (bulk or single cells)
  • Data analysis (including unsupervised, supervised, high-dimensional)

Services

  • Access to flow cytometry analyzers and a flow cytometry sorter
  • Advice on multi-color experimental design and data interpretation
  • Personal assistance for analysis available by appointment
  • Extensive training on analyzers
  • Training and education in flow cytometry
  • High-end, supervised cell sorting

Fields of research

  • Immunology/Hematology
  • Genetic and metabolic diseases
  • Cell biology
  • Molecular biology
  • Microbiology
  • Infectious diseases
  • Gynaecology (sperm)
  • Biochemistry
  • Chemistry (small particles)

Flow cytometry Expertise and services

The Radboudumc Technology Center Flow Cytometry has extensive experience with flow cytometry in basic and translational research, as well as in patient diagnostics, follow-up and clinical trials.

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How can we help enhance your research? Contact us

Willemijn Hobo PhD
Technology center representative

+31 (0)24 361 98 40
contact

Facility Flow cytometry and equipment

Radboudumc offers access to flow cytometry analyzers and a flow cytometry sorter.

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Applications

  • Cell phenotyping
  • Standardized immune profiling
  • Cell sorting based on fluorescent proteins
  • 4-way multiple populations sorting
  • Multiwell single cell sorting in 6-384 well plates for single analysis applications
  • Nucleic acid analysis/ Cell cycle analysis
  • FRET measurements
  • Small particles analysis and sorting
  • Cell function analysis (calcium efflux)
  • Dynamic multidimensional immune functionality profiling

Using our equipment

Users who have operated flow cytometry equipment will, after evidence of proficiency, be allowed to use the equipment unassisted. Individual and group training in the use of our equipment is available to allow for unassisted use in the future.

see instructions

Using our equipment

Where

NIG Building M379 room number 4.148 (route number 475).

When

Mondays through Fridays from 8:00 to 18:00 hrs.

Reservations flow cytometry analysers

Click here to reserve via the booking platform.

Reservations for sorting

You can make a reservation request using your own Outlook agenda and invite the cell sorter.
  • In outlook select calendar (agenda) and then new (nieuw)
  • Select guide card invite attendees (deelnemers uitnodigen)  and send to (aan) Vz apparatuur Laboratoriumgeneeskunde LH flowcytometer Aria (VZ00329) and add this to required (vereist).
  • You can make this appointment. Select scheduling (planning). The bars indicate when the cell sorter is busy. Select the time you need to sort you samples.
  • Select appointment (afspraak). Mention your name and phone number under subject (onderwerp).
  • Finally, press send (verzenden).

Training

Contact Head Operator Rob Woestenenk at +31 (0)24 361 52 32 for instructions.

What is flow cytometry?

Flow cytometry is a sophisticated technique of single cell analysis.

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What is flow cytometry?

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Flow cytometry is a sophisticated technique of single cell analysis. It simultaneously measures and analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. Some of the characteristics measured include a particle's relative size, granularity or internal complexity and relative fluorescence intensity.


  • A flow cytometer is composed of three main systems: fluidics, optics and electronics. The fluidics transports the particles in a stream to the laser beam for interrogation. The optics consists of lasers to illuminate the particles in the sample stream and optical filters to direct the resulting light signals to the appropriate detectors. The electronics convert the detected light signals into digital signals that can be processed by the computer.

    Monoclonal antibodies tagged with a fluorescent dye can be used to identify a particular cell type based on individual surface, cytoplasmic, or nuclear antigens. Different mixtures of fluorochromes can be used to distinguish sub-populations. Dyes, which bind directly to cell components such as DNA and RNA, are used as well.

    The flow technology provides a powerful and sensitive tool for investigating the structure and function of cells, bacteria and other particles. These measurements can be made at flow rates of 100-20.000 cells per second.

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