The Radboud Biobank (RB) and the laboratory of Medical Immunology (LMI) are making plans to replace the density gradient centrifugation method with a new automated PBMC isolation method, the AutoMACS Pro Separator.
In summary, the yield of PBMCs after isolation with the AutoMACS procedure was similar or greater in comparison to the density gradient procedure, while viability and the phenotypic distribution after both isolation procedures was comparable.
All studies that collect PBMCs have already been informed by mail and after agreement the RB will plan a start date with LMI, a new SOP will be written and coupled to the samples in the RB. For more information or questions on this new PBMC isolation method contact the Radboud Biobank.
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