8 April 2021

Ruben Smeets, theme Inflammatory diseases, and colleagues published in Autoimmunity their data which showed high concordance of prevalent systemic sclerosis (SSc)-specific autoantibodies between different diagnostic assays. Further standardisation for low prevalent SSc-specific and SSc-associated autoantibodies is needed.


Autoimmune antibody profiling plays a prominent role in both classification and prognosis of SSc. In the last years novel autoantibodies have been discovered and have become available in diagnostic assays. However, standardization in autoimmune serology is lacking, which may have a negative impact on the added value of autoantibodies in diagnosis and prognosis of SSc. In this paper we describe the comparison of commercially available diagnostic assays for the detection of SSc-associated autoantibodies and explored the coexistence of multiple SSc-associated autoantibodies within patients.


Serum samples of 347 patients from the Nijmegen Systemic Sclerosis Cohort were included in this study. All patients fulfilled the ACR/EULAR 2013 classification criteria for SSc and were classified as DcSSc or LcSSc according to the Leroy and Medsger criteria. All samples were evaluated on standard laboratory diagnostic tests for detection of SSc-specific autoantibodies CENPA and CENPB (ACA), Scl-70 (ATA), RNA Polymerase III (rp11/155) (ARA), and SSc-associated autoantibodies Fibrillarin, Th-To, PM-scl75, PM-Scl100, RNP68/A/C, Ku, NOR90, and PDGFR from suppliers EUROIMMUN, D-tek and Thermo Fisher Scientific.


We found that 79% of the patients was positive for one or more of the SSc autoantibodies. Overall, a high agreement was observed between the diagnostic methods for the SSC-specific autoantibodies listed in the ACR/EULAR criteria (ATA, ACA, and ARA) (Cohen's kappa 0.53-0.97). However, a lower agreement was found for SSc-associated autoantibodies PM-Scl, and Ku, as well as for the SSc-specific autoantibodies fibrillarin and Th-To. Furthermore, the data revealed that the presence of ATA, ARA and ACA is predominantly mutually exclusive, with only a fraction of the patients testing positive for both ATA and ARA.

Fig. Autoantibodies and association with pathophysiological profiles in Scleroderma

read more

Related news items

Joyce Aarts and colleagues published in Scientific Reports about the role of TGF-β1 in (experimental) arthritis

30 March 2022 Joyce Aarts, theme Inflammatory diseases, and her colleagues published in Scientific Reports, that local inhibition of TGF-β1 signaling improves Th17/Treg balance but not joint pathology during experimental arthritis. read more

Large NWA ORC grant awarded for national skin research: Next Generation ImmunoDermatology

23 March 2022 Research for better treatment methods for chronic skin diseases. read more

RIMLS awards call for nominations

19 October 2021 RIMLS awards several prizes to stimulate and honor our (young) researchers. Upcoming awards are Supervisor of the Year, Best Master Thesis, Best Publication, Best Image and more. Send your nominations now before 24 November 2021. read more

Best presentation award for Felicitas Pardow at the European Epidermal Barrier Research Network

28 September 2021 During the annual meeting of the European Epidermal Barrier Research Network (E2BRN) recently, Felicitas Pardow received the best presentation award. read more

ZonMw Gender in Research competition awarded to Genderful Research World Consortium

27 July 2021 Natalia Valdrighi and Esmeralda Blaney Davidson won the competition to create a plan for the dissemination of the knowledge and skills gained during the Gender in Research workshop amongst their peers, colleagues and institution, financed by ZonMw and CIHR-IGH in Canada. read more