The Radboud Biobank (RB) and the laboratory of Medical Immunology (LMI) are making plans to replace the density gradient centrifugation method with a new automated PBMC isolation method, the AutoMACS Pro Separator.
In summary, the yield of PBMCs after isolation with the AutoMACS procedure was similar or greater in comparison to the density gradient procedure, while viability and the phenotypic distribution after both isolation procedures was comparable.
All studies that collect PBMCs have already been informed by mail and after agreement the RB will plan a start date with LMI, a new SOP will be written and coupled to the samples in the RB. For more information or questions on this new PBMC isolation method contact the Radboud Biobank.
-
Want to know more about these subjects? Click on the buttons below for more news.
Related news items

Process and time constraints for external sample issuance
6 May 2025Prior to issuing samples intended for an external applicant, the Radboud Biobank first draws up a quotation of the expected costs. This process will lead to some delays in distribution.
read more
Periodic invoicing biobank collections
6 May 2025As of this year, the Radboud Biobank will periodically charge biobank collections for the processing and storage costs incurred.
read more
National Biobanks & Collections Dag 2025
6 May 2025The second edition of the National Biobanks & Collections Day took place on March 27, 2025, in Nijmegen and attracted 150 participants from all over the Netherlands.
read more
Radboud Biobank Office Relocation
30 January 2025We would like to inform you about the upcoming relocation of the Radboud Biobank office.
read more
Request for DNA samples from stored whole blood and/or saliva
30 January 2025There are biobank collections that collected whole blood and saliva before joining the Radboud Biobank to isolate DNA from them later.
read more
Great news!
30 January 2025We have good news for both new and existing collections and projects interested in isolating PBMCs (Peripheral Blood Mononuclear Cells)!
read more